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重组人血管内皮抑素联合放疗对肺腺癌A549细胞TGF-β1、HIF-1α表达的影响
Effect of Recombinant Endostatin in Combination with Radiotherapy on the Expression of TGF-β1 and HIF-1α in Lung Adenocarcinoma Cell A549

DOI: 10.12677/ACRPO.2012.12002, PP. 5-10

Keywords: 重组人血管内皮抑素;放射治疗;转化生长因子-β1;乏氧诱导因子-1α;肺腺癌
Endostar
, Radiotherapy, Transforming Growth Factor-Beta 1, Hypoxia Induced Factor-1α, Lung Adenocarcinoma

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Abstract:

通过观察Endostar联合放疗对肺腺癌A549细胞增值及TGF-β1HIF-1α表达的影响,初步探讨重组血管内皮抑素的放疗增敏机制。方法:将培养至对数生长期的A549细胞分为对照组、单纯放疗组、恩度组、联合组,检测各组A549细胞增殖率及TGF-β1HIF-1α蛋白和mRNA表达情况。结果:1) 除对照组外,各组细胞的增殖随时间的改变都受到不同程度的抑制,以联合组最为明显,差异具有统计学意义(p < 0.05)2) 处理后各组A549细胞TGF-β1HIF-1α的分泌下降,24 h以放疗组及联合组TGF-β1HIF-1α表达量明显下降

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