A New RP - HPLC Method for the Simultaneous Estimation of Lamivudine, Zidovudine and Nevirapine in Tablet Dosage Forms

An accurate, precise and reproducible high performance liquid chromatographic method was developed for the simultaneous estimation of lamivudine, zidovudine and nevirapine in pharmaceutical dosage forms. Phenomenex C18 column (250 x 4.6 mm; 5μ) was employed for theseparation of drugs. A mixture of 0.1% ortho phosphoric acid buffer (6.0 pH) and acetonitrile in the ratio of 70:30 v/v was used as the mobile phase and pumped at a flow rate of 1 mL/min. The detection wavelength was set at 268 nm. The linearity of quantification was observed in the range of 15-225, 30-450 and 20-300 μg/mL for lamivudine, zidovudine and nevirapine respectively. The proposed method was validated according to ICH guidelines. The method was found to be suitable for simultaneous and accurate determination ofthese drugs in tablet dosage forms without any interference from the excipients.