A Research on the Protective Effect of Para-Amino Salicylic Acid (PAS) on the Disadvantages of Manganese Intoxication in Kidney in Male Rats

Introduction: Concentrating of Mn in the human body leads to Manganism, a Parkinson-like disease with pathologic effects especially on brain. Being exposed to the occupations related to metals such as working in the mines, industries and chemical fertilizers in agriculture may cause manganism. Chelating agents have been used successfully in the treatment of severe manganism. This study was conducted to explore the capability of Para Amino Salicylic acid (PAS) in reducing Mn concentrations in body fluids and tissues of mn-exposed animals. Methods: Twenty vistar male rats (260-280g) were divided into four groups (control, Mn, PAS, Mn+PAS takers). Mn animals received i.p. 8mg/kg manganese chloride for one week as intoxication and PAS was injected by 1cc of 1.5g/l PAS for four weeks as the treatment. Serum, urine and kidney tissue of animals were collected for biochemical and histopathological evaluation. Results: Mn exposure significantly increased the concentration of Mn in serum and urine (p<0.001) as well as in kidney extract(p<0.05). Following PAS treatment, Mn levels in serum and kidney extract reduced, whereas in urine increased. Serum urea (P<0.05) level increasing in all test groups indicated kidney destruction in them. Higher levels of cystatin C in PAS group animals serum (p<0.01) revealed side effects on kidneys that was treated by Mn consumption. Lowering the blood and protein occurrence in urine of animals that received Mn+PAS indicated the therapeutic effects of PAS. Benefits of PAS consumption was clear in histological assays especially in the kidney cortex. Conclusion: Although PAS can reduce Mn concentration in animal's body and pathologic effects, it reveals some side effects.