Development of time-resolved laser-induced fluorescence spectroscopic technique for the analysis of biomolecules

Our developments of the time-resolved laser-induced fluorescence (TR-LIF) detection system for biomolecules are presented. This system is based on the tunable (320 nm to 475 nm) Nd:YAG laser pulses used to excite various biomolecules. The detection part is the Streak System for Fluorescence Lifetime Spectroscopy (Hamamatsu, Japan). The system consists of a C4334-01 streakscope, as a detector, DG 535 digital pulse/delay generator, C5094-S Spectrograph and HPD-TA System, as a temporal analyzer. The TR-LIF spectrometer is designed primarily to study the temperature and pressure effects on fluorescence behavior of biomolecules upon excitation with a single nanosecond pulse. The design of this system has capability to combine laser-induced breakdown (LIB) with fluorescence, as well to study optodynamic behavior of fluorescence biomolecules.