Purifica o de bromelina dos resíduos de abacaxi (Ananas comosus L. Merril) por sistemas bifásicos aquosos PEG/Sal

Due to the dimension of worldwide production of pineapple and to high amount of generated waste from its cultivation and processing, several research have been developed to recovery the enzymes presents on pineapple, such as bromelain. Bromelain is the general name given to the group of endopeptidases found in pineapple as well as all proteases derived from members of the family Bromeliaceae, which belongs to the pineapple. Because of that play important physiological roles, these enzymes offers a wide range of proven therapeutic applications. This work aims the purification of bromelain from pineapple waste through PEG/Salt aqueous two-phase systems (ATPS) using (NH4)2SO4, CaCl2 and potassium phosphate. The results showed that in CaCl2 experiments have no ATPS formation in the range of its constituents. However, the PEG/phosphate systems and PEG/(NH4)2SO4 systems showed excellent results, recovering with bromelain purification factor of 7.4 and 10.7 times, respectively, and yields above 80%.