LACTIC ACID BACTERIAL SCREENING FROM GASTROINTESTINAL DIGESTIVE TRACT OF NATIVE AND BROILER CHICKEN FOR PROBIOTIC CANDIDATE PURPOSES

The aim of his research was to obtain lactic acid bacteria (LAB) from gastrointestinal digestive tract (GIT) of chickens for probiotic candidate purposes. LAB was isolated from GIT of broiler and native chickens on selective medium (MRS+0.2% CaCO3). Screening method based on microbiological and biochemical characteristics, antibacterial properties, growth on various temperature, aeration, and agitation, antibiotic sensitivity, and viability on acid pH, gastric juice and bile salt. Thirty nine of LAB isolates was selected from native chicken and 18 isolates from broiler chicken. The selected LAB inhibited Escherichia coli FNCC 0091 growth and grown on 30, 39 and 45oC of temperature, aerobic, anaerobic and agitation conditions. Biochemical identification using API 50 CHL kit revealed that I72 from native chicken ileum as Lactobacillus salivarius and Db9 from broiler chicken duodenum as Pediococcus pentosaceus. All LAB were resistant to Erythromicin, Penicillin G and Streptomycin as tested antibiotics. Both of them have non significantly different of viability on acid pH (1, 2 and 3), gastric juice pH 2 and bile salt which were 91.78% for L. salivarius I72 and 94.48% for P. pentosaceus Db9 (P<0.05). Based on characteristics, both the selected LAB have potentiality as chicken probiotic candidates.