THE EFFECT OF GLYCEROL CONCENTRATION IN TRIS GLUCOSE EGG YOLK EXTENDER ON THE QUALITY OF TIMOR DEER FROZEN SEMEN

The aims of study was to compare the glycerol concentration in Tris glucose egg yolk (TGEY) diluents on the quality of deer frozen semen. Semen was collected from 5 Timor deer using electroejaculator. Immediately after collection the semen was evaluated macroscopic and microscopically. After initial evaluation, the semen was divided into three tubes and extended with Tris egg yolk with three different glycerol concentrations, which were 10% (TGEY10); 12% (TGEY12) and 14% (TGEY14). The sperm motility, viability, acrosome intact and membrane intact were evaluated in raw semen, after equilibration and after thawing. The results showed that there were no differences (p>0.05) on the sperm motility, viability as well as sperm acrosome intact. Sperm membrane intact in TGEY10 (52.50±5.89%) and TGEY14 (51.50±4.12 %) were higher (p<0.05) than in TGEY12 (49.00±6.58). It was concluded that 10, 12 or 14% glycerol concentration can be used for Timor deer semen cryopreservation.