INDICES OF OXIDATIVE STRESS IN SUBJECTS WITH DIFFERENT LEVELS OF BODY COMPOSITION AND TACK THE PRACTICE OF PHYSICAL ACTIVITY

ANTUNES NETO, J. M. F.; PAULA, L. B. Indices of oxidative stress in subjects with different levels of body composition and tack the practice of physical activity.Brazilian Journal of Biomotricity, v. 5, n. 2, p. 117-131, 2011. The reactive oxygen species (ROS) are derived from exogenous sources or produced endogenously, as a normal consequence of cellular functions. The unbalance caused by the excess in the production of these species associated with the fall in the cellular antioxidant capacity provokes what we call oxidative stress; a process that generates, over all, alterations in the cellular membranes and in the genes structures, at extreme circumstances. The objective of this work consisted in investigating the oxidative stress potential in sedentary obese subjects, since the obesity became a public health problem, over all in industrialized countries. Thirty men volunteers (age 26,6 ± 3,5) participated of the study and have divided in three groups, in accordance with corporal composition and active style of life: sedentary group with normal body mass index (BMI) (IMC-NS), n = 10; sedentary group with elevated BMI (IMC-ES), n = 10; and physical activity practitioner group with normal BMI (IMC-NPAF), n = 10. The analyses have divided in antropometrics – BMI, arterial pressure, waist-hip relation and fat percentage; biochemistry – hemoglobin dosage; antioxidant markers – catalase (CAT) and total sulphydril groups (TSG); oxidant markers – thiobarbituric acid reactive substances (TBARs); and cellular alteration markers – creatine kinase (CK). The results had shown that the subjects of IMC-ES group, when compared to the other groups, have lower levels of CAT (0,19 ± 0,10 k/gHb/min), TSG (298,0 ± 67,0 μM), TBARS (1,60 ± 0,40 nmol/mL) and CK (45,0 ± 25,0 U/L), probably for the sedentary condition where if they find. The subjects of IMC-NS group, although also sedentary condition, had presented significantly bigger values of CAT (0,30 ± 0,04 k/gHb/min, p<0,05), TSG (450,0 ± 111,0 μM, p<0,01), TBARS (2,30 ± 1,0 nmol/mL, p<0,05) and CK (100 ± 25, p<0.05), in relation to obese, suggesting that the physical condition can determine the cellular regulatory adjustments. In relation to IMC-NPAF group, the significant increases of CAT (0,40 ± 0,10 k/gHb/min, p<0,01), TSG (503,0 ± 121,0 μM, p<0,01), TBARS (2,80 ± 1,40 nmol/mL, p<0,05) and CK (150,0 ± 45,0 U/L, p<0,05), when compared to IMC-ES group, reflects the influence of the physical activity in the adaptive answers. In conclusion, the oxidative stress levels can be more dangerous to the sedentary obese subjec