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Cells  2013 

Imaging and Quantitation Techniques for Tracking Cargo along Endosome-to-Golgi Transport Pathways

DOI: 10.3390/cells2010105

Keywords: optical imaging, super-resolution microscopy, flow cytometry, Golgi apparatus, endosomes, fluorescent proteins

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Abstract:

Recent improvements in the resolution of light microscopy, coupled with the development of a range of fluorescent-based probes, have provided new approaches to dissecting membrane domains and the regulation of membrane trafficking. Here, we review these advances, as well as highlight developments in quantitative image analysis and novel unbiased analytical approaches to quantitate protein localization. The application of these approaches to endosomal sorting and endosome-to-Golgi transport is discussed.

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