Enzymatic in-situ generation of H2O2 for decolorization of Acid Blue 113 by fenton process

Decolorization of Acid Blue 113 in an aqueous medium by bio-Fenton process has been investigated in this research. Enzymatic oxidation of glucose was performed to in-situ generation of H2O2 which was employed to react with Fe2+ for producing hydroxyl radicals. The effect of various parameters include concentrations of 113, glucose, and FeSO4, activity of glucose oxidase (GOx) and the effect of pH were assessed. The highest decolorization of AB 113 were achieved at Fe2+ concentration of 0.2 mmol/L, pH =4.0, glucose concentration of 0.018 mol/L, and glucose oxidase activity of 2500 U/L in the constant temperature (23 ±0.1oC) and constant shaking rate (160 r/min), while the concentration of 113 was 40 mg/L. In these conditions, 113 decolorization efficiency after 60 min was obtained about 95%.