Responses of Chrysichthys furcatus to glyphosate formulation and Garcinia kola seed extracts were investigated using the organization for Economic Cooperation and Development numbers 203 and 407 recommended toxicity bioassay. The fish were divided into five groups and exposed to different treatments of glyphosate formulation and Garcinia kola seed extract, with the control serving as a reference. Water quality parameters and blood chemistry were estimated in the experimental and control fish at the end of 28 days. There was no significant difference ( ) between fish treated with glyphosate formulation alone and other treatments except for dissolved oxygen which was highly significant ( ) between fish treated with glyphosate formulation alone and other treatments and the control. All the blood parameters were significantly ( ) affected by glyphosate formulation when compared with the control. The changes observed in glyphosate formulation alone were reversible when G. kola seeds extract was added and was dose dependent. The plant’s extract has shown to be a good remedy to pollutants, and formulations of the seed extract into tablets or capsules could serve as antidote to ameliorate the effects of pollutants. This finding can reduce the risk of biomagnifications of poisons along the food chain. 1. Introduction In the last few years there has been an exponential growth in the field of herbal medicine and these drugs are gaining popularity both in developing and developed countries because of their natural origin and less side effects [1]. Herbal products cause few adverse effects but have beneficial pharmacological and therapeutic uses in a number of illnesses, including HIV where they have been examined for their capacity to reduce symptoms and improve quality of life [2]. The literature review had shown that oral administration of an aqueous T. cordifolia root extract to alloxan diabetic rats caused a significant reduction in blood glucose and brain lipids. Though the aqueous extract at a dose of 400？mg/kg could elicit significant antihyperglycemic effect in different animal models, its effect was equivalent to only one unit/kg of insulin [3]. It is reported that the daily administration of either alcoholic or aqueous extract of T. cordifolia decreases the blood glucose level and increases glucose tolerance in rodents [4]. Also, oral administration of 2 and 8？g/kg of plant extract of fenugreek seeds produced dose dependent decrease in the blood glucose levels in both normal diabetic rats [5]. The plant’s seed extract also improved glucose metabolism and
References
[1]
J. K. Grover, S. Yadav, and V. Vats, “Medicinal plants of India with anti-diabetic potential,” Journal of Ethnopharmacology, vol. 81, no. 1, pp. 81–100, 2002.
[2]
R. Weber, L. Christen, M. Loy et al., “Randomized, placebo-controlled trial of Chinese herb therapy for HIV-1- infected individuals,” Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology, vol. 22, no. 1, pp. 56–64, 1999.
[3]
K. S. Dhaliwal, “Inventor. Method and composition for treatment of diabetes,” US Patent. 5886029. 1999, 1999.
[4]
S. S. Gupta, S. C. Verma, V. P. Garg, and M. Rai, “Anti-diabetic effects of Tinospora cardifolia. I. Effect on fasting blood sugar level, glucose tolerance and adrenaline induced hyperglycaemia,” The Indian Journal of Medical Research, vol. 55, no. 7, pp. 733–745, 1967.
[5]
P. Ravikumar and C. V. Anuradha, “Effect of fenugreek seeds on blood lipid peroxidation and antioxidants in diabetic rats,” Phytotherapy Research, vol. 13, pp. 197–201, 1999.
[6]
P. Khosla, D. D. Gupta, and R. K. Nagpal, “Effect of Trigonella foenum graecum (Fenugreek) on blood glucose in normal and diabetic rats,” Indian Journal of Physiology and Pharmacology, vol. 39, no. 2, pp. 173–174, 1995.
[7]
K. Regi Raphael, M. C. Sabu, and R. Kuttan, “Hypoglycemic effect of methanol extract of Phyllanthus amarus Schum & Thonn on alloxan induced diabetes mellitus in rats and its relation with antioxidant potential,” Indian Journal of Experimental Biology, vol. 40, no. 8, pp. 905–909, 2002.
[8]
U. J. Okoli, An Investigation into the Hypoglycemic Activity of GB1 Biflavonoids of Garcinia Kola, B. Pharma Project, University of Nigeria, Nsukka, Nigeria, 1991.
[9]
O. O. Ebong and T. Korubo-Owiye, “Comparison of the effect of the seeds Garcinia kola on the gastric acid secretion in rats,” West African Journal of Pharmacology and Drug Research, vol. 12, pp. 51–54, 1996.
[10]
G. C. Onunkwo, H. C. Egeonu, M. U. Adikwu, J. E. Ojile, and A. K. Olowosulu, “Some physical properties of tabletted seed of Garcinia kola (Heckel),” Chemical and Pharmaceutical Bulletin, vol. 52, no. 6, pp. 649–653, 2004.
[11]
K. A. Oluyemi, I. O. Omotuyi, O. R. Jimoh, O. A. Adesanya, C. L. Saalu, and S. J. Josiah, “Erythropoietic and anti-obesity effects of Garcinia cambogia (bitter kola) in Wistar rats,” Biotechnology and Applied Biochemistry, vol. 46, no. 1, pp. 69–72, 2007.
[12]
M. M. Mackeen, A. M. Ali, N. H. Lajis, K. Kawazu, H. Kikuzaki, and N. Nakatani, “Antifungal garcinia acid esters from the fruits of Garcinia atroviridis,” Zeitschrift fur Naturforschung C, vol. 57, no. 3-4, pp. 291–295, 2002.
[13]
M. M. Iwu, O. A. Igboko, O. K. Elekwa, and M. S. Tempesta, “Prevention of thioacetamide-induced hepatotoxicity by biflavanones of Garcinia kola,” Phytotherapy Research, vol. 4, no. 4, pp. 157–159, 1990.
[14]
S. B. Olaleye and E. O. Farombi, “Analgesic and Ati inflammatory effects of Kolaviron,” African Journal of Biomedical Research, vol. 3, pp. 171–174, 2000.
[15]
V. B. Braide, C. A. Agube, G. E. Essien, and F. V. Udoh, “Effect of Garcinia kola seed alkaloid extract on levels of gonadal hormones and pituitary gonadotrophins in rat serum,” Nigerian Journal of Physiological Sciences, vol. 18, pp. 59–64, 2003.
[16]
A. O. Akpantah, A. A. Oremosu, C. C. Moronhna, J. B. Ekanem, and A. O. Okanlawon, “Effect of Garcinia kola seed extracts on ovulation, oestrous cycle, and foetal Development in Cyclic Sprague Dawley Rats,” Nigerian Journal of Physiological Sciences, vol. 20, no. 1-2, pp. 58–562, 2005.
[17]
N. Gunsel and J. F. Kanig, “Tablets,” in Theory and of Industrial Pharmacy, L. Lachman, H. A. Liberman, J. L. Kanig, et al., Eds., Lea and Febiger, Philadelphia, Pa, USA, 2nd edition, 1976.
[18]
L. Graves, “Roundup: Birth Defects Caused by World's Top-Selling weedkiller,” 2011.
[19]
R. A. Relyea, “The impact of insecticides and herbicides on the biodiversity and productivity of aquatic communities,” Ecological Applications, vol. 15, no. 2, pp. 618–627, 2005.
[20]
J. A. Springett and R. A. J. Gray, “Effect of repeated low doses of biocides on the earthworm Aporrectodea caliginosa in laboratory culture,” Soil Biology and Biochemistry, vol. 24, no. 12, pp. 1739–1744, 1992.
[21]
S. A. Hassan, F. Bigler, H. Bogenschütz et al., “Results of the fifth joint pesticide testing programme carried out by the IOBC/WPRS-Working Group ‘Pesticides and beneficial organisms’,” Entomophaga, vol. 36, no. 1, pp. 55–67, 1991.
[22]
A. Paganelli, V. Gnazzo, H. Acosta, S. L. López, and A. E. Carrasco, “Glyphosate-based herbicides produce teratogenic effects on vertebrates by impairing retinoic acid signaling,” Chemical Research in Toxicology, vol. 23, no. 10, pp. 1586–1595, 2010.
[23]
J. Doublet, L. Mamy, and E. Barriuso, “Delayed degradation in soil of foliar herbicides glyphosate and sulcotrione previously absorbed by plants: consequences on herbicide fate and risk assessment,” Chemosphere, vol. 77, no. 4, pp. 582–589, 2009.
[24]
E. Chivian and A. Bernstein, in Threatened Groups of Organisms Valuable to MedicineSustaining Life: How Human Health Depends on Biodiversity, E. Chivian, Ed., p. 209, Oxford University Press, New York, NY, USA, 2008.
[25]
J. P. Giesy, S. Dobson, and K. R. Solomon, “Ecotoxicological risk assessment for Roundup herbicide,” Reviews of Environmental Contamination and Toxicology, vol. 167, pp. 35–120, 2000.
[26]
J. Salbego, A. Pretto, C. R. Gioda et al., “Herbicide formulation with glyphosate affects growth, acetylcholinesterase activity, and metabolic and hematological parameters in Piava (leporinus obtusidens),” Archives of Environmental Contamination and Toxicology, vol. 58, no. 3, pp. 740–745, 2010.
[27]
O. A. Oyelese and E. O. Faturoti, “Growth and mortality estimates in Clarias gariepinus fed graded levels of processed cassava peels,” Journal of Tropical Forest Resources, vol. 11, pp. 71–81, 1995.
[28]
OECD (Organization for Economic Cooperation and Development), Guideline for the Testing of Chemicals, Fish, acute toxicity test, No. 203, 1992.
[29]
APHA, AWWA, WEF, Standard Methods for the Examination of Water and Waste Water, American Public Health Association, Washington, DC, USA, 20th edition, 1998.
[30]
D. L. Reish and O. S. Oshida, “Manual of methods in aquatic environment research. Part 10. Short- term static Bioassays,” FAO Fisheries Technical Paper 247, Rome, Italy, 1987.
[31]
D. J. Finney, Probit Analysis, Cambridge University Press, Cambridge, UK, 1971.
[32]
OECD, “OECD Guideline for the Testing of Chemicals 407, ’’Reapeted Dose 28-day Oral Toxicity Study in Rodent,” adopted on July 1995, 1997.
[33]
C. E. Boyd and C. S. Tucker, Water Quality and Pond Soil Analysis for Aquaculture, vol. 1014, Alabama Agricultural Experiment Station, Auburn University, Auburn, Ala, USA, 1992.
[34]
J. L. Congleton and W. J. LaVoie, “Comparison of blood chemistry values for samples collected from juvenile Chinook salmon by three methods,” Journal of Aquatic Animal Health, vol. 13, pp. 168–172, 2001.
[35]
V. Rusia and S. K. Sood, “Routine haematological test,” in Medical Laboratory Technology, K. L. Mukerjee, Ed., pp. 252–258, Tata McGraw Hill Publishing, 1992.
[36]
H. Bomski, Postawowe Laboratoryjne Badania Hematologiczne. (Basic Laboratory Techniques in Haematology), PZWL, Warsaw, Poland, 1995 (polish).
[37]
D. L. Drabkin, “Spectrophotometric studies, XIV, The crystallographic and optimal properties of the haemoglobin of man in comparison with those of other species,” Journal of Biological Chemistry, vol. 164, pp. 703–723, 1946.
[38]
P. B. Goldenfarb, F. P. Bowyer, E. Hall, and E. Brosious, “Reproducibility in the hematology laboratory: the microhematocrit determination,” American Journal of Clinical Pathology, vol. 56, no. 1, pp. 35–39, 1971.
[39]
L. R. Murrell and P. F. Nace, “Determination of glucose in fish blood; a modification of the Folin-Malmros microprocedure,” Canadian Journal of Biochemistry and Physiology, vol. 36, no. 11, pp. 1121–1124, 1958.
[40]
P. Trinder, “Determination of blood glucose using an oxidase-peroxidase system with a non-carcinogenic chromogen,” Journal of Clinical Pathology, vol. 22, no. 2, pp. 158–161, 1969.
[41]
O. H. Lowry, N. J. Rosebrough, A. L. Farr, and R. J. Randall, “Protein measurement with the Folin phenol reagent,” The Journal of Biological Chemistry, vol. 193, no. 1, pp. 265–275, 1951.
[42]
G. Barseghian, R. Levine, and P. Epps, “Direct effect of cortisol and cortisone on insulin and glucagon secretion,” Endocrinology, vol. 111, no. 5, pp. 1648–1651, 1982.
[43]
S. K. Chiu, C. P. Collier, A. F. Clark, and K. E. Wynn-Edwards, “Salivary cortisol on ROCHE Elecsys immunoassay system: pilot biological variation studies,” Clinical Biochemistry, vol. 36, no. 3, pp. 211–214, 2003.
[44]
A. Petrie and P. Watson, Statistics for Veterinary and Animal Science, vol. 1, Blackwell Science, Oxford, UK, 1st edition, 1999.
[45]
R. A. Fisher, Statistical Methods for Research Workers, Oliver and Boyd, London, UK, 11th edition, 1950.
[46]
A. K. Sinha, V. P. Singh, and K. Srivastava, “Physico-chemical studies on river Ganga and its tributaries in Uttar Pradesh-the present status,” in Pollution and Biomonitoring of Indian Rivers, R. K. Trevedi, Ed., ABD publishers, Jaipur, India, 2000.
[47]
A. M. Kalwale and A. Padmakar, “Determination of physico-chemical parameters of Deoli Bhorus dam water,” Advances in Applied Science Res, vol. 3, pp. 273–279, 2012.
[48]
M. A. Haniffa and S. M. Vijayarani, “Hematological effects of textile mill effluent on freshwater fish Oreochromis mossambicus (Trewaves),” Indian Journal of Experimental Biology, vol. 27, no. 5, pp. 476–478, 1989.
[49]
M. Z. Vosyliene and N. Kazlauskiene, “Evaluation of the Svede pond water effect on fish (after accidental discharge of the Kairiai dump filtrate into the environment) and management of water bodies,” in Proceedings of the International Scientific Conference, pp. 219–223, Kaunas, 2004.
[50]
T. O. Ikpesu, Responses of Clarias gariepinus and Oreochromis niloticus to endosulfan [Ph.D. thesis], Department the Department of Animal and Environmental Biology, University of Benin Nigeria, 2010.
[51]
N. Seth and K. K. Saxena, “Hematological responses in a freshwater fish Channa punctatus due to fenvalerate,” Bulletin of Environmental Contamination and Toxicology, vol. 71, no. 6, pp. 1192–1199, 2003.
[52]
“Fresh water,” in Handbook of Ecotoxicology, P. Calins, Ed., vol. 68 of Osneymeed OX 20EL, p. 73, Black Well Science, 1995.
[53]
Z. Svobodova, D. Ravds, and J. Palackova, Unified Methods of Haematological Examination of Fish, Research Institute of Fish Culture and Hydrobiology, Vodňany, Czech Republic, 1991.
[54]
V. Lusková, M. Svoboda, and J. Kolá？ová, “The effect of diazinon on blood plasma biochemistry in carp (Cyprinus carpio L.),” Acta Veterinaria Brno, vol. 71, no. 1, pp. 117–123, 2002.
[55]
J. Nemcsok and L. Boross, “Comparative studies on the sensitivity of different fish species to metal pollution,” Acta Biologica Academiae Scientiarum Hungaricae, vol. 33, no. 1, pp. 23–27, 1982.
[56]
G. Wedemeyer, D. J. Mcleay, and C. P. Good year, “Assessing the tolerance of fish and fish population to environmental stress. The problems and methods of monitoring,” in Contaminant Effects on Fisheries, W. V. Cairns, P. V. Hodson, and J. O. Nriagu, Eds., pp. 164–195, John Wiley and Son, New York, NY, USA, 1981.
[57]
N. N. Sing and A. K. Srivastava, “Effects of endosulfan on fish carbohydrate metabolism,” Ecotoxicology and Environmental Safety, vol. 5, pp. 412–417, 1981.
[58]
R. C. Andrews and B. R. Walker, “Glucocorticoids and insulin resistance: old hormones, new targets,” Clinical Science, vol. 96, no. 5, pp. 513–523, 1999.
[59]
E. Omoregie, E. B. C. Ofodike, and R. I. Keke, “Tissue chemistry of Oreochromis niloticus exposed to sublethal concentration of gammalin-20 and acetellic -25EC,” Journal of Aquatic Science, vol. 5, pp. 33–36, 1990.
[60]
S. R. Sharma, S. K. Dwivedi, and D. Swarup, “Hypoglycaemic, antihyperglycaemic and hypolipidemic activities of Caesalpinia bonducella seeds in rats,” Journal of Ethnopharmacology, vol. 58, no. 1, pp. 39–44, 1997.
[61]
E. H. Karunanayake, J. Welihinda, S. R. Sirimanne, and G. Sinnadorai, “Oral hypoglycaemic activity of some medicinal plants of Sri Lanka,” Journal of Ethnopharmacology, vol. 11, no. 2, pp. 223–231, 1984.
[62]
A. O. Aderibigbe, T. S. Emudianughe, and B. A. Lawal, “Antihyperglycemic effect of Mangifera indica in rat,” Phytotherapy Research, vol. 13, pp. 504–507, 1999.
[63]
B. A. Shibib, L. A. Khan, and R. Rahman, “Hypoglycaemic activity of Coccinia indica and Momordica charantia in diabetic rats: depression of the hepatic gluconeogenic enzymes glucose-6-phosphatase and fructose-1,6-bisphosphatase and elevation of both liver and red-cell shunt enzyme glucose-6-phosphate dehydrogenase,” Biochemical Journal, vol. 292, no. 1, pp. 267–270, 1993.
[64]
F. Jenkins, J. Smith, B. Rajanna et al., “Effect of sub-lethal concentrations of endosulfan on hematological and serum biochemical parameters in the carp Cyprinus carpio,” Bulletin of Environmental Contamination and Toxicology, vol. 70, no. 5, pp. 993–997, 2003.
[65]
M. Takahashi and D. A. Hood, “Protein import into subsarcolemmal and intermyofibrillar skeletal muscle mitochondria: differential import regulation in distinct subcellular regions,” Journal of Biological Chemistry, vol. 271, no. 44, pp. 27285–27291, 1996.
[66]
S. M. Kamble, P. L. Kamlakar, S. Vaidya, and V. D. Bambole, “Influence of Coccinia indica on certain enzymes in glycolytic and lipolytic pathway in human diabetes,” Indian Journal of Medical Sciences, vol. 52, no. 4, pp. 143–146, 1998.
[67]
J. Du, Y. Wang, R. Hunter et al., “Dynamic regulation of mitochondrial function by glucocorticoids,” Proceedings of the National Academy of Sciences of the United States of America, vol. 106, no. 9, pp. 3543–3548, 2009.
[68]
R. L？sel and M. Wehling, “Nongenomic actions of steroid hormones,” Nature Reviews Molecular Cell Biology, vol. 4, no. 1, pp. 46–56, 2003.
[69]
S. Pathak, A. Banerjee, S. Paul, and A. R. Khuda-Bukhsh, “Protective potentials of a plant extract (Lycopodium clavatum) on mice chronically fed hepato-carcinogens,” Indian Journal of Experimental Biology, vol. 47, no. 7, pp. 602–607, 2009.
