Background. In most hospitals of developing countries, urinary tract infections are treated empirically because of lack of culture facilities. This leads to emergence of multiresistant uropathogens. Culturing and drug susceptibility testing are essential to guide therapy. Objectives. To assess changing prevalence and resistance pattern of uropathogens to commonly used antibiotics in a two-year study period. Methods. Urine specimens were collected and cultured. Uropathogens were identified by standard methods and tested for antibiotics resistance. Data were analyzed using SPSS version 16 statistical sofware. P value < 0.05 was considered statistically significant. Results. The commonest isolates in both the previous and present studies were E. coli, Klebsiella, CoNS, S. aureus, Proteus, and Citrobacter species. Previous isolates of Enterobacteriaceae were 100% sensitive to ciprofloxacin, whereas present isolates developed 31% to 60% resistance to it. Previous isolates were less resistant to gentamycin than the present ones. Multiresistance isolates were predominant in present study than previous ones. Conclusion. E. coli was predominant in the two study periods. Present isolates were more resistant than previous ones. Some previous isolates were 100% sensitive to ciprofloxacin, whereas present isolates were increasingly resistant. Ciprofloxacin and gentamicin have been recommended for empiric treatment of urinary tract infections. 1. Background Urinary tract infection is one of the commonest bacterial infections encountered in daily clinical practice [1]. It has been estimated that worldwide about 150 million people suffer from asymptomatic and symptomatic UTIs each year [2]. In most parts of the sub-Saharan Africa, as well as in other developing parts of the world, UTI is among the most common health problems occurring both in the community and hospitalized patients [3]. Since the last two to three decades, just as many community and hospital acquired bacterial infections, UTIs due to multidrug resistant uropathogens have caused a growing concern worldwide [1, 4–6]. Investigators [1, 7, 8] explained that the drug resistance problem in Africa stems from factors like indiscriminate use of antibiotics, inappropriate advertisement, and erratic prescription by unqualified drug sellers. Since the previous two decades, the problem of UTIs due to uropathogens resistant to the commonly used antibiotics was reported by many authors in Ethiopia in general and in Gondar region in particular [1, 9–12]. Consequently, the prevalence of urinary tract pathogens and
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