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In Vitro Screening for Abiotic Stress Tolerance in Potent Biocontrol and Plant Growth Promoting Strains of Pseudomonas and Bacillus spp.

DOI: 10.1155/2014/195946

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Abstract:

Plant growth promoting rhizobacteria (PGPR) has been identified as a group of microbes that are used for plant growth enhancement and biocontrol for management of plant diseases. The inconsistency in performance of these bacteria from laboratory to field conditions is compounded due to the prevailing abiotic stresses in the field. Therefore, selection of bacterial strains with tolerance to abiotic stresses would benefit the end-user by successful establishment of the strain for showing desired effects. In this study we attempted to isolate and identify strains of Bacillus and Pseudomonas spp. with stress tolerance and proven ability to inhibit the growth of potential phytopathogenic fungi. Screening of bacterial strains for high temperature (50°C), salinity (7% NaCl), and drought (?1.2?MPa) showed that stress tolerance was pronounced less in Pseudomonas isolates than in Bacillus strains. The reason behind this could be the formation of endospores by Bacillus isolates. Tolerance to drought was high in Pseudomonas strains than the other two stresses. Three strains, P8, P20 and P21 showed both salinity and temperature tolerance. P59 strain possessed promising antagonistic activity and drought tolerance. The magnitude of antagonism shown by Bacillus isolates was also higher when compared to Pseudomonas strains. To conclude, identification of microbial candidate strains with stress tolerance and other added characteristic features would help the end-user obtain the desired beneficial effects. 1. Introduction A wide range of agriculturally important microorganisms (AIMs) have been exploited for crop health management, which comprise nitrogen fixers like Rhizobium, Bradyrhizobium, Sinorhizobium, Azotobacter, Azospirillum, phosphate solubilisers like Bacillus, Pseudomonas, Aspergillus, and arbuscular mycorrhizae (AM); and fungi, bacteria, viruses and nematodes used for pest and disease management in agriculture, horticulture, and forestry. Plant growth promoting rhizomicroorganisms (PGPR) is known to increase plant growth and induce host plant resistance and crop yield [1]. As the crops are affected by abiotic stresses such as soil moisture deficit stress, high temperature, soil salinity, and so forth, microbes are also known to be affected by these conditions. Reports from Madhya Pradesh and Chhattisgarh of India indicated that the free living rhizobial population declines to lower than the minimum threshold levels required for nodulation due to high soil temperatures requiring inoculation every year [2, 3]. Widden and Hsu [4] observed that the ability of

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