A simple, sensitive, and accurate RP-HPLC coupled with UV detector method was developed and validated for simultaneous determination of matrine and tinidazole in compound lotion. The chromatographic separation of the two compounds was carried out with a SinoChoom ODS-BP C18 column (5？μm, 4.6？mm？×？200？mm) analytical column, using a mobile phase consisting of 0.025？mol/L potassium dihydrogen phosphate (containing triethylamine 0.05%, v/v) and acetonitrile (80？:？20, v/v) at a flow rate of 1.0？mL/min. The detection was monitored at 210 and 310？nm for matrine and tinidazole, respectively. Total run time was 12？min, and the column was maintained at 25°C. The excipients in the compound lotion did not interfere with the drug peaks. The calibration curves of matrine and tinidazole were fairly linear over the concentration ranges of 10.0–100.0？μg/mL ( ) and 20.0–200.0？μg/mL ( ), respectively. The RSD of both the intraday and interday variations was below 1.5% for matrine and tinidazole. The proposed HPLC method was validated according to International Conference on Harmonisation and proved to be suitable for the simultaneous determination of matrine and tinidazole in compound lotion. 1. Introduction Vaginosis is an inflammation of the vagina. It can result in discharge, itching, and pain and is often associated with an irritation or infection of the vulva. The infection is usually caused by Candida albicans, Gardnerella, Trichomonas vaginalis, chlamydia, and Mycoplasma. It is estimated that bacterial vaginosis is associated with a twofold increased risk of preterm birth and a sixfold increased risk of miscarriage [1]. Bacterial vaginosis is also found to be associated with an increased susceptibility to sexually transmitted infections, herpes simplex viruses, human papillomavirus, and human immunodeficiency virus [2, 3]. It is necessary to prepare an effective preparation for prevention and treatment of vaginosis. Sophora flavescens Ait is a leguminous plant widely distributed in the northwestern region of China. It is an important Chinese herbal remedy that has been used for thousands of years in history. The important ingredient extracted from the herbal remedy was matrine whose chemical name is (7aS, 13aR, 13bR, 13cS)-dodecahydro-1H, 5H, 10H-dipyrido ] [ ] naphthyridin-10-one (Figure 1) [4, 5]. It has been officially listed in the Chinese Pharmacopoeia. Previous studies have indicated that matrine has a variety of pharmacological effects, including anti-inflammatory, anticancer, and action as a kappa opioid receptor and μ-receptor agonist [6–9]. It was often
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