Purpose. This study examines the effects of fundus fluorescein angiography (FFA) procedure on ocular pulse amplitude (OPA) and intraocular pressure (IOP). Materials and Methods. Sixty eyes of 30 nonproliferative diabetic retinopathy patients (15 males, 15 females) were included in this cross-sectional case series. IOP and OPA were measured with the Pascal dynamic contour tonometer before and after 5 minutes of intravenous fluorescein dye injection. Results. Pre-FFA mean OPA value was ？mmHg and post-FFA mean OPA value was ？mmHg ( ). Pre-FFA mean IOP value was ？mmHg and post-FFA mean IOP value was ？mmHg ( ). Conclusion. Although both mean OPA and IOP values were decreased after FFA procedure, the difference was not statistically significant. This clinical trial is registered with Australian New Zealand Clinical Trials Registry number ACTRN12613000433707. 1. Introduction Fundus fluorescein angiography (FFA) is a procedure for the examination of chorioretinal circulation. It can be used to confirm the diagnosis, monitor the progress of posterior segment diseases, and assess the efficacy of certain treatments like laser photocoagulation and intravitreal injections. Although FFA is a safe procedure, some serious complications like allergic skin rash and anaphylaxis could be seen rarely [1]. The effect of fluorescent molecule or FFA procedure on choroidal circulation is not known much. The dynamic contour tonometry (DCT) is a noninvasive and direct intraocular pressure (IOP) measuring device and is considered to accurately measure the IOP independent of the corneal thickness or corneal elasticity [2]. It also provides a continuous examination of the IOP and measures ocular pulse amplitude (OPA) which is accepted as an indirect measurement of the choroidal blood flow [3]. The OPA is generally considered as the difference between systolic and diastolic values of the pulsatile IOP. It gives us an opinion about the choroidal blood flow corresponding with the heart pulse as a function of time [4]. Choroidal circulation has a vital role in ocular physiology and may be affected in various ocular and systemic diseases [5, 6]. FFA is an imaging procedure using fluorescence ability, and it has been thought to have little or no effect on ocular physiology. The rationale of this study was that fluorescein sodium passes freely through the choroidal vessels into the extravasal space and this would have an influence on choroidal blood flows, either by an osmotic effect or a hemorheological effect. Also, influence of fluorescein sodium on the erythrocyte aggregation was shown
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