This study presents three simple, rapid, and accurate spectrophotometric methods for the determination of Rasagiline (RSG) in pharmaceutical preparations. The determination procedures depend on the reaction of RSG with chloranilic acid for method A, tetrachloro-1,4-benzoquinone for method B, and 7,7,8,8-tetracyanoquinodimethane for method C. The colored products were quantitated spectrophotometrically at 524, 535, and 843？nm for methods A, B, and C, respectively. Different variables affecting the reaction were optimized. Linearity ranges of the methods with good correlation coefficients (0.9988–0.9996) were observed as 25–300？μg？mL？1, 25–350？μg？mL？1, and 50–500？μg？mL？1 for methods A, B, and C, respectively. The formation of products takes place through different mechanisms. The sites of interaction were confirmed by elemental analysis using IR and 1H-NMR spectroscopy. The validation of the methods was carried out in terms of specificity, linearity, accuracy, precision, robustness, limit of detection, and limit of quantitation. No interference was observed from concomitants usually present in dosage forms. The methods were applied successfully to the determination of RSG in pharmaceutical preparations. 1. Introduction In Parkinson’s disease, one of the most prominent pathologies is the progressive degeneration of melanin-containing neurons in the substantia nigra pars com-pacta, resulting in the depletion of nigrostriatal dopamine [1]. Rasagiline (RSG) is a potent selective irreversible inhibitor of monoamine oxidase type B (2) and has been used for the treatment of idiopathic Parkinson’s disease [2]. The reported analytical methods for determination of RSG include high-performance liquid chromatography (HPLC) [3–6], spectrophotometry [7, 8], and gas chromatography combined with mass spectrometry (GC-MS) [9]. No spectrophotometric method using π-acceptors was reported in the literature. π-acceptors such as chloranilic acid (CA), tetrachloro-1,4-benzoquinone (p-chloranil), and 7,7,8,8-tetracyanoquinodimethane (TCNQ) are known to yield charge transfer (CT) complexes and radical anions with a variety of electron donors [10–15]. The spectrophotometric methods, based on these interactions, are usually simple and applicable for drug substances. Therefore, the aim of the present study was directed to investigate simple, rapid, sensitive, and cost-effective spectrophotometric methods based on the reactions using π-acceptors. The developed analytical procedures depend on the reaction of RSG with CA is expressed as method A, with p-chloranil is expressed as method
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