全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...
Diversity  2013 

Genetic Diversity and Seed Quality of the “Badda” Common Bean from Sicily (Italy)

DOI: 10.3390/d5040843

Keywords: landrace, on-farm conservation, phaseolin, Phaseolus vulgaris, seed quality, SSR

Full-Text   Cite this paper   Add to My Lib

Abstract:

The genetic structure of the “Badda” common bean cultivated at Polizzi Generosa, a village of Sicily (Palermo, Italy), was investigated using biochemical and molecular markers. Seed storage protein analysis by using SDS-PAGE, confirmed the attribution to the Andean gene pool. Simple Sequence Repeats (SSR) (or microsatellite) molecular markers provided useful information on genetic variation and relationships between “Badda bianco” and “Badda nero” morphotypes. Based on SSR data, the nine accessions examined were grouped in three sub-clusters. The first sub-cluster included all the accessions belonging to the “Badda bianco”. Conversely, “Badda nero” was constituted by two well-distinguished sub-clusters, one of them forming a well-separated branch. This result suggests that two constitutive nuclei contributed to the genetic background of “Badda nero”. Moreover, technological and nutritional data evidenced a good seed protein content (mean value 240.7 g kg ? 1) and differences in seed hydration rate among accessions. Knowledge of genetic structure appear to be fundamental in planning safeguard strategies of an appreciate landrace such as the “Badda” bean.

References

[1]  Piergiovanni, A.R.; Lioi, L. Italian common bean landraces: History, genetic diversity and seed quality. Diversity 2010, 2, 837–862, doi:10.3390/d2060837.
[2]  Escribano, M.R.; Santalla, M.; Casquero, P.A.; de Ron, A.R. Patterns of genetic diversity in landraces of common bean (Phaseolus vulgaris L.) from Galicia. Plant Breed. 1998, 117, 49–56, doi:10.1111/j.1439-0523.1998.tb01447.x.
[3]  Rodi?o, A.P.; Santalla, M.; Montero, I.; Casquero, P.A.; de Ron, A.M. Diversity of common bean (Phaseolus vulgaris L.) germplasm from Portugal. Genet. Resour. Crop Evol. 2001, 48, 409–417, doi:10.1023/A:1012248002436.
[4]  Mavromatis, A.G.; Arvanitoyannis, I.S.; Korkovelos, A.E.; Giakountis, A.; Chatzitheodorou, V.A.; Goulas, C.K. Genetic diversity among common bean (Phaseolus vulgaris L.) Greek landraces and commercial cultivars: Nutritional components, RAPD and morphological markers. Span. J. Agric. Res. 2010, 8, 986–994.
[5]  Ganopoulos, I.; Bosmali, I.; Madesis, P.; Tsaftari, A. Microsatellite genotyping with HRM (High Resolution Melting) analysis for identification of the PGI common bean variety Plake Megalosperma Prespon. Eur. Food Res. Technol. 2012, 234, 501–508, doi:10.1007/s00217-011-1653-z.
[6]  Montesano, V.; Negro, D.; Sarli, G.; Logozzo, G.; Spagnoletti Zeuli, P.L. Landraces in inland of the Basilicata region, Italy: Monitoring and perspectives for on farm conservation. Genet. Resour. Crop Evol. 2012, 59, 701–716, doi:10.1007/s10722-011-9712-7.
[7]  Negri, V.; Tosti, N. Phaseolus genetic diversity maintained on-farm in central Italy. Genet. Resour. Crop Evol. 2002, 49, 511–520, doi:10.1023/A:1020902508166.
[8]  Angioi, S.A.; Rau, D.; Rodriguez, M.; Logozzo, G.; Desiderio, F.; Papa, R.; Attene, G. Nuclear and chloroplast microsatellite diversity in Phaseolus vulgaris L. from Sardinia (Italy). Mol. Breed. 2009, 23, 413–429, doi:10.1007/s11032-008-9245-8.
[9]  Mercati, F.; Leone, M.; Lupini, A.; Sorgonà, A.; Bacchi, M.; Abenavoli, M.R.; Sunseri, F. Genetic diversity and population structure of a common bean (Phaseolus vulgaris L.) collection from Calabria (Italy). Genet. Resour. Crop. Evol. 2013, 60, 839–852, doi:10.1007/s10722-012-9879-6.
[10]  Paniconi, G.; Gianfilippi, F.; Mosconi, P.; Mazzuccato, A. Distinctiveness of bean landraces in Italy: The case study of the “Badda” bean. Diversity 2010, 2, 701–716, doi:10.3390/d2050701.
[11]  Lioi, L.; Nuzzi, A.; Campion, B.; Piergiovanni, A.R. Assessment of genetic variation in common bean (Phaseolus vulgaris L.) from Nebrodi mountains (Sicily, Italy). Genet. Resour. Crop Evol. 2012, 59, 455–464, doi:10.1007/s10722-011-9696-3.
[12]  Tredicesima revisione dell’elenco dei prodotti agroalimentari tradizionali. Available online: http://www.politicheagricole.it/flex/cm/pages/ServeBLOB.php/L/IT/IDPagina/3276 (accessed on 4 October 2013).
[13]  Fondazione Slow Food. I presidi Slow Food in Italia. Available online: http://www.fondazioneslowfood.it/pagine/ita/presidi_italia/dettaglio_presidi.lasso?-id=3545 (accessed on 4 October 2013).
[14]  Comune di Polizzi Generosa. Available online: http://www.comune.polizzi.pa.it/index.php?option=com_alberghi&Itemid=130&task=viewcategory&catid=68 (accessed on 4 October 2013).
[15]  Gepts, P.; Bliss, F.A. Dissemination pathways of common bean (Phaseolus vulgaris, Fabaceae) deduced from phaseolin electrophoretic variability. II. Europe and Africa. Econ. Bot. 1988, 42, 86–104, doi:10.1007/BF02859038.
[16]  Maciel, F.L.; Echeverrigaray, S.; Gerald, L.T.S.; Grazziotin, G.F. Genetic relationships and diversity among Brazilian cultivars and landraces of common bean (Phaseolus vulgaris L.) revealed by AFLP markers. Genet. Resour. Crop Evol. 2003, 50, 887–893, doi:10.1023/A:1025994513043.
[17]  Blair, M.W.; Giraldo, M.C.; Buendia, H.F.; Tovar, E.; Duque, M.C.; Beebe, S.E. Microsatellite marker diversity in common bean (Phaseolus vulgaris L.). Theor. Appl. Genet. 2006, 113, 100–109, doi:10.1007/s00122-006-0276-4.
[18]  Grisi, M.C.; Blair, M.W.; Gepts, P.; Brondani, C.; Pereira, P.A.; Brondani, R.P. Genetic mapping of a new set of microsatellite markers in a reference common bean (Phaseolus vulgaris) population BAT93 x Jalo EEP558. Genet. Mol. Res. 2007, 30, 691–706.
[19]  Lioi, L.; Piergiovanni, A.R.; Pignone, D.; Puglisi, S.; Santantonio, M.; Sonnante, G. Genetic diversity of some surviving on-farm Italian common bean (Phaseolus vulgaris L) landraces. Plant Breed. 2005, 124, 576–581, doi:10.1111/j.1439-0523.2005.01153.x.
[20]  Bollini, R.; Chrispeels, M.J. Characterization and subcellular localization of vicilin and phytohemagglutinin, the two major reserve proteins of Phaseolus vulgaris L. Planta 1978, 142, 291–298, doi:10.1007/BF00385080.
[21]  Paz, M.M.; Veilleux, R.E. Genetic diversity based on randomly amplified polymorphic DNA (RAPD) and its relationship with the performance of diploid potato hybrids. J. Am. Soc. Hort. Sci. 1997, 122, 740–747.
[22]  Association of Official Agricultural Chemists (AOAC). Official Methods of Analysis, 11th ed. ed.; AOAC: Washington, DC, USA, 1970.
[23]  Piergiovanni, A.R.; Cerbino, D.; della Gatta, C. Diversity in seed quality traits of common bean (Phaseolus vulgaris L.) populations from Basilicata (Southern Italy). Plant Breed. 2000, 119, 513–516, doi:10.1046/j.1439-0523.2000.00531.x.
[24]  Nei, M. Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics 1978, 89, 583–590.
[25]  Yeh, F.C.; Yang, R.C.; Boyle, T. POPGENE. version 1.32; Microsoft Window-based freeware for population genetic analysis. Available online: http://www.ualberta.ca/~fyeh/popgene_download.html (accessed on 4 December 2013).
[26]  Takezaki, N.; Nei, M.; Tamura, K. POPTREE2: Software for constructing population trees from allele frequency data and computing other population statistics with windows interface. Mol. Biol. Evol. 2010, 27, 747–752, doi:10.1093/molbev/msp312.
[27]  Lioi, L. Geographical variation of phaseolin pattern in an old world collection of Phaseolus vulgaris. Seed Sci. Technol. 1989, 17, 317–324.
[28]  Ocampo, C.H.; Martin, J.P.; Sanchez-Yelamo, M.D.; Ortiz, J.M.; Toro, O. Tracing the origin of Spanish common bean cultivars using biochemical and molecular markers. Genet. Resour. Crop Evol. 2005, 52, 33–40, doi:10.1007/s10722-005-1931-3.
[29]  Sicard, D.; Nanni, L.; Porfiri, O.; Bulfon, D.; Papa, R. Genetic diversity of Phaseolus vulgaris L. and P. coccineus L. landraces in central Italy. Plant Breed. 2005, 124, 464–472, doi:10.1111/j.1439-0523.2005.01137.x.
[30]  Bellucci, E.; Bitocchi, E.; Rau, D.; Rodriguez, M.; Biagetti, E.; Giardini, A.; Attene, G.; Nanni, L.; Papa, R. Genomics of Origin, Domestication and Evolution of Phaseolus vulgaris. In Genomics of Plant Genetic Resources; Tuberosa, R., Graner, A., Frison, E., Eds.; Springer: Dordrecht, The Netherlands, 2014; Volume 1, pp. 483–507.
[31]  Angioi, S.A.; Rau, D.; Nanni, L.; Bellucci, E.; Papa, R.; Attene, G. The genetic make-up of the European landraces of the common bean. Plant Genet. Resour. 2011, 9, 197–201, doi:10.1017/S1479262111000190.
[32]  Logozzo, G.; Donnoli, R.; Macaluso, L.; Papa, R.; Knupffer, H.; Spagnoletti Zeuli, P.L. Analysis of the contribution of Mesoamerican and Andean gene pools to European common bean (Phaseolus vulgaris L.) germplasm and strategies to establish a core collection. Genet. Resour. Crop Evol. 2007, 54, 1763–1779, doi:10.1007/s10722-006-9185-2.
[33]  Perazzini, R.; Leonardi, D.; Ruggeri, S.; Alesiani, D.; D’Arcangelo, G.; Canini, A. Characterization of Phaseolus vulgaris L. landraces cultivated in central Italy. Plant Foods Hum. Nutr. 2008, 63, 211–218, doi:10.1007/s11130-008-0095-7.
[34]  Limongelli, G.; Laghetti, G.; Perrino, P.; Piergiovanni, A.R. Variation of seed storage proteins in landraces of common bean (Phaseolus vulgaris L.) from Basilicata, southern Italy. Euphytica 1996, 92, 393–399.
[35]  Piergiovanni, A.R.; Maruca, G.; Lioi, L. Biochemical Characterization of Common Bean Germplasm Maintained On-Farm in Calabria Region. In Proceedings of the IX Convegno Nazionale sulla Biodiversità, Valenzano, Italy, 5–7 September 2012.
[36]  Brown, J.W.S.; Osborn, T.C.; Bliss, F.A.; Hall, T.C. Bean lectins. Part 1: Relationships between agglutinating activity and electrophoretic variation in the lectin-containing G2/albumin seed proteins of French bean (Phaseolus vulgaris L.). Theor. Appl. Genet. 1982, 62, 263–271.
[37]  Lioi, L. Electrophoretic variation and geographical distribution of the seed protein phytohemagglutinin in cultivated Phaseolus vulgaris L. J. Genet. Breed. 1991, 45, 97–102.
[38]  Blair, M.W.; González, L.F.; Kimani, P.M.; Butare, L. Genetic diversity, inter-gene pool introgression and nutritional quality of common beans (Phaseolus vulgaris L.) from Central Africa. Theor. Appl. Genet. 2010, 121, 237–248, doi:10.1007/s00122-010-1305-x.
[39]  Burle, M.L.; Fonseca, J.R.; Kami, J.A.; Gepts, P. Microsatellite diversity and genetic structure among common bean (Phaseolus vulgaris L.) landraces from Brazil, a secondary center of diversity. Theor. Appl. Genet. 2010, 12, 801–813.

Full-Text

Contact Us

[email protected]

QQ:3279437679

WhatsApp +8615387084133