Presence of Viral Genome in Urine and Development of Hematuria and Pathological Changes in Kidneys in Common Marmoset (Callithrix jacchus) after Inoculation with Dengue Virus
Common marmosets ( Callithrix jacchus) developed high levels of viremia, clinical signs including fever, weight loss, a decrease in activity and hematuria upon inoculation with dengue virus (DENV). Presence of DENV genome in urine samples and pathological changes in kidneys were examined in the present study. Levels of DENV genome were determined in 228 urine samples from 20 primary DENV-inoculated marmosets and in 56 urine samples from four secondary DENV-inoculated marmosets. DENV genome was detected in 75% (15/20) of marmosets after primary DENV infection. No DENV genome was detected in urine samples from the marmosets with secondary infection with homologous DENV (0%, 0/4). Two marmosets demonstrated hematuria. Pathological analysis of the kidneys demonstrated non-suppressive interstitial nephritis with renal tubular regeneration. DENV antigen-positive cells were detected in kidneys. In human dengue virus infections, some patients present renal symptoms. The results indicate that marmosets recapitulate some aspects of the involvement of kidneys in human DENV infection, and suggest that marmosets are potentially useful for the studies of the pathogenesis of DENV infection, including kidneys.
References
[1]
Kuo, M.C.; Lu, P.L.; Chang, J.M.; Lin, M.Y.; Tsai, J.J.; Chen, Y.H.; Chang, K.; Chen, H.C.; Hwang, S.J. Impact of renal failure on the outcome of dengue viral infection. Clin. J. Am. Soc. Nephrol. 2008, 3, 1350–1356, doi:10.2215/CJN.00020108.
Omatsu, T.; Moi, M.L.; Hirayama, T.; Takasaki, T.; Nakamura, S.; Tajima, S.; Ito, M.; Yoshida, T.; Saito, A.; Katakai, Y.; Akari, H.; Kurane, I. Common marmoset (Callithrix jacchus) as a primate model of dengue virus infection: Development of high levels of viraemia and demonstration of protective immunity. J. Gen. Virol. 2011, 92, 2272–2280, doi:10.1099/vir.0.031229-0.
[4]
Omatsu, T.; Moi, M.L.; Takasaki, T.; Nakamura, S.; Katakai, Y.; Tajima, S.; Ito, M.; Yoshida, T.; Saito, A.; Akari, H.; Kurane, I. Changes in hematological and serum biochemical parameters in common marmosets (Callithrix jacchus) after inoculation with dengue virus. J. Med. Primatol. 2012, 41, 289–296, doi:10.1111/j.1600-0684.2012.00552.x.
[5]
Hirayama, T.; Mizuno, Y.; Takeshita, N.; Kotaki, A.; Tajima, S.; Omatsu, T.; Sano, K.; Kurane, I.; Takasaki, T. Detection of dengue virus genome in urine by real-time reverse transcriptase PCR: A laboratory diagnostic method useful after disappearance of the genome in serum. J. Clin. Microbiol. 2012, 50, 2047–2052, doi:10.1128/JCM.06557-11.
