Arabinoxylan (AX) is a major dietary fibre component found in a variety of cereals. Numerous health benefits of arabinoxylans have been reported to be associated with their solubility and molecular features. The current study reports the development of a functional bread using a combination of AX-enriched material (AEM) and optimal commercial endoxylanase. The total AX content of bread was increased to 8.2 g per 100 g available carbohydrates. The extractability of AX in breads with and without endoxylanase was determined. The results demonstrate that water-extractable AX (WE-AX) increased progressively through the bread making process. The application of endoxylanase also increased WE-AX content. The presence of 360 ppm of endoxylanase had positive effects on the bread characteristics in terms of bread volume and firmness by converting the water unextractable (WU)-AX to WE-AX. In addition, the molecular weight (Mw) distribution of the WE-AX of bread with and without endoxylanase was characterized by size-exclusion chromatography. The results show that as the portion of WE-AX increased, the amount of high Mw WE-AX (higher than 100 kDa) decreased, whereas the amount of low Mw WE-AX (lower than 100 kDa) increased from 33.2% to 44.2% through the baking process. The low Mw WE-AX further increased to 75.5% with the application of the optimal endoxylanase (360 ppm).
References
[1]
Anderson, W.J.; Baird, P.; Davis, H.R., Jr.; Ferreri, S.; Knudtson, M.; Koraym, A.; Waters, V.; Williams, L.C. Health benefits of dietary fiber. Nutr. Rev. 2009, 67, 188–205, doi:10.1111/j.1753-4887.2009.00189.x.
Broekaert, W.F.; Courtin, C.M.; Verbeke, K.; van de Wiele, T.; Verstraete, W.; Delcour, J.A. Prebiotic and other health-related effects of cereal-derived arabinoxylans, arabinoxylan-oligosaccharides, and xylooligosaccharides. Crit. Rev. Food Sci. Nutr. 2011, 51, 178–194, doi:10.1080/10408390903044768.
Fran？ois, I.E; Lescroart, O.; Veraverbeke, W.S.; Marzorati, M.; Possemiers, S.; Evenepoel, P.; Hamer, H.; Houben, E.; Windey, K.; Welling, G.W.; et al. Effects of a wheat bran extract containing arabinoxylan oligosaccharides on gastrointestinal health parameters in healthy adult human volunteers: A double-blind, randomised, placebo-controlled, cross-over trial. Br. J. Nutr. 2012, 108, 2229–2242.
[6]
Toole, G.A.; le Gall, G.; Colquhoun, I.J.; Johnson, P.; Bedo, Z.; Saulnier, L.; Shewry, P.R.; Mills, E.N.C. Spectroscopic analysis of diversity of Arabinoxylan structures in endosperm cell walls of wheat cultivars (Triticum aestivum) in the HEALTHGRAIN diversity collection. J. Agric. Food Chem. 2011, 59, 7075–7082, doi:10.1021/jf201095m.
[7]
Damen, B.; Pollet, A.; Dornez, E.; Broekaert, W.F.; Haesendonck, I.V.; Trogh, I.; Arnaut, F.; Delcour, J.A.; Courtin, C.M. Xylanase-mediated in situ production of arabinoxylan oligosaccharides with prebiotic potential in whole meal breads and breads enriched with arabinoxylan rich material. Food Chem. 2012, 131, 111–118, doi:10.1016/j.foodchem.2011.08.043.
[8]
Lu, Z.X.; Walker, K.Z.; Muir, J.G.; Mascara, T.; O’Dea, K. Arabinoxylan fiber, a byproduct of wheat flour processing, reduces the postprandial glucose response in normoglycemic subjects. Am. J. Clin. Nutr. 2000, 71, 1123–1128.
[9]
Lu, Z.X.; Walker, K.Z.; Muir, J.G.; O’Dea, K. Arabinoxylan fibre improves metabolic control in people with Type II diabetes. Eur. J. Clin. Nutr. 2004, 58, 621–628, doi:10.1038/sj.ejcn.1601857.
[10]
Zhou, S.; Liu, X.; Guo, Y.; Wang, Q.; Peng, D.; Cao, L. Comparison of the immunological activities of arabinoxylans from wheat bran with alkali and xylanase-aided extraction. Carbohydr. Polym. 2010, 81, 784–789, doi:10.1016/j.carbpol.2010.03.040.
[11]
Cao, L.; Liu, X.Z.; Qian, T.X.; Sun, G.B.; Guo, Y.; Chang, F.J.; Zhou, S.M.; Sun, X.B. Antitumor and immunomodulatory activity of arabinoxylans: A major constituent of wheat bran. Int. J. Biol. Macromol. 2011, 48, 160–164, doi:10.1016/j.ijbiomac.2010.10.014.
[12]
Cloetens, L.; Swennen, K.; de Preter, V.; Broekaert, W.F.; Courtin, C.M.; Delcour, J.A.; Rutgeerts, P.; Verbeke, K. Effect of arabinoxylo-oligosaccharides on proximal gastrointestinal motility and digestion in healthy volunteers. e-SPEN 2008, 35, e220–e225.
[13]
Grootaert, C.; Delcour, J.A.; Courtin, C.M.; Broekaert, W.F.; Verstraete, W.; Wiele van de, T. Microbial metabolism and prebiotic potency of arabinoxylan oligosaccharides in the human intestine. Trends Food Sci. Technol. 2007, 18, 64–71, doi:10.1016/j.tifs.2006.08.004.
[14]
Grootaert, C.; van den Abbeele1, P.; Marzorati1, M.; Broekaert, W.F.; Courtin, M.C.; Delcour, J.A.; Verstraete1, W.; Wiele van de, T. Comparison of prebiotic effects of arabinoxylan oligosaccharides and inulin in a simulator of the human intestinal microbial ecosystem. FEMS Microbiol. Ecol. 2009, 69, 231–242, doi:10.1111/j.1574-6941.2009.00712.x.
[15]
Maki, K.C.; Gibson, G.R.; Dickmann, R.S.; Kendall, C.W.C.; Chen, C.Y.O.; Costabile, A.; Comelli, E.M.; McKay, D.L.; Almeida, N.G.; Jenkins, D.; et al. Digestive and physiologic effects of a wheat bran extract, arabino-xylan-oligosaccharide, in breakfast cereal. Nutrition 2012, 28, 1115–1121, doi:10.1016/j.nut.2012.02.010.
[16]
U.S. Food and Drug Administration. Agency Response Letter GRAS Notice No. GRN 000343. CFSAN/Office of Food Additive Safety. Available online: http://www.fda.gov/Food/ IngredientsPackagingLabeling/GRAS/NoticeInventory/ucm235936.htm (access on 15 February 2013).
[17]
AACC Internationl. Approved Methods of Analysis, 11th Ed. Method 74-09.01. Measurement of Bread Firmness by Universal Testing Machine; AACC International: St. Paul, MN, USA, 1999.
[18]
Douglas, S.G. A rapid method for the determination of pentosans in wheat flour. Food Chem. 1980, 7, 139–145, doi:10.1016/0308-8146(81)90059-5.
[19]
Hollmann, J.; Lindhauer, M.G. Pilot-scale isolation of glucuronoarabinoxylans from wheat bran. Carbohydr. Polym. 2005, 59, 225–230, doi:10.1016/j.carbpol.2004.09.015.
[20]
Bataillon, M.; Mathaly, P.; Crdinali, A.P.N.; Duchiron, F. Extraction and purification ofarabinoxylan from destarched wheat bran in a pilot scale. Ind. Crop. Prod. 1998, 8, 37–43, doi:10.1016/S0926-6690(97)10002-4.
[21]
Courtin, C.M.; Delcour, J.A. Arabinoxylans and endoxylanases in wheat flour bread-making. J. Cereal Sci. 2002, 35, 225–243, doi:10.1006/jcrs.2001.0433.
[22]
Redgwell, R.J.; de Michieli, J.-H.; Fischer, M.; Reymond, S.; Nicolas, P.; Sievert, D. Xylanase induced changes to water- and alkaliextractable arabinoxylans in wheat flour: Their role in lowering batter viscosity. J. Cereal Sci. 2001, 33, 83–96, doi:10.1006/jcrs.2000.0345.
[23]
Skendi, A.; Biliaderis, C.G; Izydorczyk, M.S.; Zervou, M.; Zoumpoulakis, P. Structural variation and rheological properties of water-extractable arabinoxylans from six Greek wheat cultivars. Food Chem. 2011, 126, 526–536.
[24]
Courtin, C.M.; Delcour, J.A. Physicochemical and bread-making properties of low molecular weight wheat derived arabinoxylans. J. Agri. Food Chem. 1998, 46, 4066–4073, doi:10.1021/jf980339t.
[25]
Cleemput, G.; Booij, C.; Hessing, M.; Gruppen, H.; Delcour, J.A. Solubilisation and changes in molecular weight distribution of arabinoxylan and protein in wheat flours during bread-making, and the effects of endogenous arabinoxylan hydrolysing enzymes. J. Cereal Sci. 2000, 26, 55–66.
[26]
Goesaert, H.; Brijs, K.; Veraverbeke, W.S.; Courtin, C.M.; Gebruers, K.J.; Delcour, J.A. Wheat flour constituents: How they impact bread quality, and how to impact their functionality. Trends Food Sci. Technol. 2005, 16, 12–30.
[27]
Courtin, C.M.; Gelders, G.G.; Delcour, J.A. The use of two endoxylanases with different substrate selectivity provides insight into the functionality of arabinoxylans in wheat flour breadmaking. Cereal Chem. 2001, 78, 564–571, doi:10.1094/CCHEM.2001.78.5.564.
[28]
Henrissat, B.; Bairoch, A. New families in the classification of glycosyl hydrolases based on amino acid sequence similarities. Biochem. J. 1993, 293, 781–788.
[29]
Reilly, P.J. Xylanases, Structure and Function. In Trends in the Biology of Fermentation for Fuels and Chemicals; Hollaender, A., Ed.; Plenum Press: New York, NY, USA, 1981; pp. 111–129.
[30]
Li, K.; Azadi, P.; Collins, R.; Tolan, J.; Kim, J.S.; Eriksson, K.L. Relationships between activities of xylanases and xylan structures. Enzyme Microb. Technol. 2000, 27, 89–94.
[31]
Vardakou, M.; Katapodis, P.; Samiotaki, M.; Kekos, D.; Panayotou, G.; Christakopoulos, P. Mode of action of family 10 and 11 endoxylanases on water-unextractable arabinoxylan. Int. J. Biol. Macromol. 2003, 33, 129–134, doi:10.1016/S0141-8130(03)00077-1.
[32]
Pitkanen, L.; Tuomainen, P.; Virkki, L.; Tenkanen, M. Molecular characterization and solution properties of enzymatically tailored arabinoxylans. Int. J. Biol. Macromol. 2011, 49, 963–969, doi:10.1016/j.ijbiomac.2011.08.020.
[33]
Courtin, C.M.; Roelants, A.; Delcour, J.A. Fractionation-reconstitution experiments provide insight into the role of endoxylanases in bread-making. J. Agric. Food Chem. 1999, 47, 1870–1877, doi:10.1021/jf981178w.
[34]
Arnaut, F.; Willem, F.; Broekaert, W.F.; Courtin, C.; Delcour, J.A.; Georis, J.; van Haesendonck, I.P.H. Bread with Increased Arabinoxylo-Oligosaccharide Content. U.S. Patent WO2008087167 A, 24 July 2008.
[35]
Makaravicius, T.; Basinskiene, L.; Juodeikiene, G.; van Gool, M.P.; Schols, H.A. Production of oligosaccharides from extruded wheat and rye biomass using enzymatic treatment. Catal. Today 2012, 196, 16–25.
European Food Safety Authority. Scientific Opinion on the substantiation of health claims related to arabinoxylan produced from wheat endosperm and reduction of post-prandial glycaemic responses (ID 830) pursuant to Article 13(1) of Regulation (EC) No 1924/2006. EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA). EFSA J. 2011, 9, 2205.
[38]
Hughes, S.A.; Shewry, P.R.; Li, L.; Gibson, G.R.; Sanz, M.L.; Rastall, R.A. In vitro fermentation by human fecal microflora of wheat arabinoxylans. J. Agric. Food Chem. 2007, 55, 4589–4595.
[39]
Vardakou, M.; Palop, C.N.; Christakopoulos, P.; Faulds, C.B.; Gasson, M.A.; Narbad, A. Evaluation of the prebiotic properties of wheat arabinoxylan fractions and induction of hydrolase activity in gut microflora. Int. J. Food Microbiol. 2008, 123, 166–170, doi:10.1016/j.ijfoodmicro.2007.11.007.
