The present study is an attempt to investigate oil and grease pollution that may pollute fresh water and influence aquatic environment. Then removal of oil and grease from manufacturing wastewater befall essential but common techniques not enough. Enzyme and adsorption units representing major developed new laboratory were selected to assess the water quality and humiliation prospective of oil and grease from wastewater. Several components and environmental variables that were dissolved oxygen, bacteriology measure, flow rate and adsorption material amount studied to assess the removal performance of oil and grease. The results elucidated significant variations among different tests which influenced microbial necessary role of oxidation declining develop biological treatment process reached to 72%. The study stressed out natural material (zeolite) that enhanced organic reduction under optimal conditions. These conditions were closer spacing and high length of adsorbing unit that led to increase oil and grease contact period with adsorbent and added to increase performance removal reached to 99%. 1. Introduction Organic toxic waste (oil and grease (O&G)) causes ecology damages for aquatic organisms [1], plant, animal, and equally, mutagenic and carcinogenic for human being [2]. They discharge from different sources to form a layer on water surface that decreases dissolved oxygen. O&G layer reduces biological activity of treatment process where oil film formation around microbes in suspended matter and water. This lead to decrease dissolved oxygen levels in the water. Then oxygen molecules are difficulty to be oxidative for microbial on hydrocarbon molecules and cause ecology damages to water bodies [3, 4]. The conventional techniques remove oil and grease using skimming tanks and oil and grease traps in treatment plants but the main disadvantage of these methods is their low efficiency of removal. The remaining oil causes clogging of pipes in treatment units that need cleaning and sometimes replacement of pipes. This lead to increase maintenance and inspection cost [5]. Recently, alternative uses of biochemical route (enzymes and lipases) have potentially gained more attention due to their clean and friendly application and to overcome limitation [6]. Microbial activity plays significant role in performance, strength purification process, and elimination of pretreatment process in wastewater treatment plant depending on enzyme costs [7]. Environmental studies described prevention of fat blockage or filming in waste systems before discharging wastewater
References
[1]
M. S. Islam, M. Saiful, M. Hossain, M. Sikder, M. Morshed, and M. Hossain, “Acute toxicity of the mixtures of grease and engine wash oil on fish, pangasius sutch, under laboratory condition,” International Journal Life Science, Biotechnology and Pharmacology Research, vol. 2, no. 1, pp. 306–317, 2013.
[2]
W. U. Lan, G. E. Gang, and W. A. N. Jinbao, “Biodegradation of oil wastewater by free and immobilized Yarrowia lipolytica W29,” Journal of Environmental Sciences, vol. 21, no. 2, pp. 237–242, 2009.
[3]
T. J. Alade, A. M. Suleyman, M. L. Abdul Karim, and M. Z. Alam, “Removal of oil and grease as Emerging Pollutants of Concern (EPC) in wastewater stream,” IIUM Engineering Journal, vol. 12, no. 4, pp. 161–169, 2011.
[4]
S. Facchin, P. D. D. Alves, S. F. de Faria, M. B. Tatiana, M. N. V. Júnia, and K. Evanguedes, “Biodiversity and secretion of enzymes with potential utility in wastewater treatment,” Journal of Ecology, vol. 3, no. 1, pp. 34–47, 2013.
[5]
S. A. Mueller, B. R. Kim, J. E. Anderson, A. Gaslightwala, M. J. Szafranski, and W. A. Gaines, “Removal of oil and grease and chemical oxygen demand from oily automotive wastewater by adsorption after chemical de-emulsification,” Practice Periodical of Hazardous, Toxic, and Radioactive Waste Management, vol. 7, no. 3, pp. 156–162, 2003.
[6]
M. C. M. R. Leal, D. M. G. Freire, M. C. Cammarota, and G. L. Sant'Anna Jr., “Effect of enzymatic hydrolysis on anaerobic treatment of dairy wastewater,” Process Biochemistry, vol. 41, no. 5, pp. 1173–1178, 2006.
[7]
D. Alberton, D. A. Mitchell, J. Cordova, P. Peralta-Zamora, and N. Krieger, “Production of a fermented solid containing lipases of Rhizopus microsporus and its application in the pre-hydrolysis of a high-fat dairy wastewater,” Food Technology and Biotechnology, vol. 48, no. 1, pp. 28–35, 2010.
[8]
Environmental Oasis, “WW07P-Grease removal and food processing,” 2012, http://www.oasisenviro.co.uk/ww07pproductinfo.html.
[9]
R. Bussamara, A. M. Fuentefria, E. S. D. Oliveira et al., “Isolation of a lipase-secreting yeast for enzyme production in a pilot-plant scale batch fermentation,” Bioresource Technology, vol. 101, no. 1, pp. 268–275, 2010.
[10]
E. Rigo, R. E. Rigoni, P. Lodea, D. D. Oliveira, D. M. G. Freire, and M. D. Luccio, “Application of different lipases as pretreatment in anaerobic treatment of wastewater,” Environmental Engineering Science, vol. 25, no. 9, pp. 1243–1248, 2008.
[11]
Y. Zhang, B. Cui, S. Wang et al., “Relation between enzyme activity of sediments and lake eutrophication in grass-type lakes in North China,” Clean—Soil, Air, Water, vol. 40, no. 10, pp. 1145–1153, 2012.
[12]
H. Horchani, I. Aissa, S. Ouertani, Z. Zarai, Y. Gargouri, and A. Sayari, “Staphylococcal lipases: biotechnological applications,” Journal of Molecular Catalysis B: Enzymatic, vol. 76, pp. 125–132, 2012.
[13]
H. K. Shon, D. Tian, D.-Y. Kwon, C.-S. Jin, T.-J. Lee, and W.-J. Chung, “Degradation of fat, oil, and grease (FOGs) by lipase-producing bacterium Pseudomonas sp. strain D2D3,” Journal of Microbiology and Biotechnology, vol. 12, no. 4, pp. 583–591, 2002.
[14]
P. N. Ibegbulam-Njoku, O. K. Achi, and C. C. Chijioke-Osuji, “Use of palm oil mill effluent as fermentative medium by lipase producing,” International Journal of Scientific & Engineering Research, vol. 5, no. 2, 2014.
[15]
W. Yeoung-Sheng, H. Shu-Huei, L. Chang-Hung, and H. Jao-Jia, “Adsorption of complex pollutants from aqueous solutions by nanocomposite materials,” Clean—Soil, Air, Water, vol. 41, no. 6, pp. 574–580, 2013.
[16]
APHA, American Public Health Association Standard Methods for the Examination of Water and Wastewater, APHA, New York, NY, USA, 22nd edition, 2012.
[17]
R. Gupta, N. Gupta, and P. Rathi, “Bacterial lipases: an overview of production, purification and biochemical properties,” Applied Microbiology and Biotechnology, vol. 64, no. 6, pp. 763–781, 2004.
[18]
L. Bora and M. C. Kalita, “Production and optimization of thermostable lipase from a thermophilic Bacillussp. LBN4,” The Internet Journal of Microbiology, vol. 4, no. 1, 2007, http://www.ispub.com/journal/the-internet-journal-of-microbiology/volume-4-number-1/production-and-optimization-of-thermostable-lipase-from-a-thermophilic-Bacillus-sp-lbn-4.html#sthash.qRHstqSc.dpbs.
[19]
S. Bradoo, R. K. Saxena, and R. Gupta, “Two acidothermotolerant lipases from new variants of Bacillus spp,” World Journal of Microbiology and Biotechnology, vol. 15, no. 1, pp. 97–102, 1999.
[20]
S. S. -Dash, R. Subramani, and D. S. Kompala, A Method for Rapid Treatment of Wastewater and a Composition Thereof, World Intellectual Property Organization (WIPO), Geneva, Switzerland, 2011.
