Introduction. Chicken anaemia virus, CAV, was until recently the only member of the Gyrovirus genus. 6 novel gyroviruses, AGV2, HGyV1, and GyV3-6, have since been discovered in human and chicken samples. Methods. PCR amplification of the VP2 gene was used to detect AGV2/HGyV1, GyV3, and CAV in a range of clinical samples including stool, respiratory, CSF, and HIV-positive plasma. Screening of fresh local chicken meat was also performed. Results. AGV2/HGyV1 or GyV3 was detected in stools from healthy children (17/49, 34.7%) and patients with diarrhoea (22/149, 14.8%). 1.2% (3/246) nasopharyngeal respiratory samples were positive. No AGV2/HGyV1 or GyV3 was detected in nasal swabs from wheezing patients, in CSF from patients with meningitis, and in HIVpositive plasma. CAV was found in 51% (25/49) of stools from healthy children and 16% (24/149) in diarrhoea samples. Screening of 28 chicken samples showed a higher prevalence of gyrovirus (20/28, 71%) compared to CAV (1/28, 3.6%). Phylogenetic analysis of the CAV VP1 gene showed South African sequences clustering with Brazilian isolates from genotypes D2 and A2. Conclusion. Novel gyroviruses, including CAV, are present in the South African population with diarrhoea and respiratory illness as well as in healthy children. Their presence suggests an origin from chicken meat consumption. 1. Introduction Until recently chicken anaemia virus (CAV) was the only member of the genus Gyrovirus in the Circoviridae family. This genus is characterized by small nonenveloped DNA viruses with a negative sense single-stranded circular DNA of about 2.3？kb [1]. Circoviruses, in contrast, have an ambisense genome. The similarity of the gyrovirus genome organization to annelloviruses, with 3 overlapping open reading frames (ORFs), has led to the recommendation that gyroviruses become a subfamily, Gyrovirinae, within the Anelloviridaefamily [2]. In early 2011 Rijsewijk et al. [3] reported the discovery of a distant relative to CAV, avian gyrovirus 2 (AGV2), in diseased chicken from Brazil, with only 40% homology to CAV. Later that year, Sauvage et al. [4] identified a very closely related gyrovirus on human skin (HGyV1). Subsequently 4 other novel gyroviruses have been described. Gyrovirus 3 (GyV3) was identified by viral metagenomics in faeces from Chilean children with acute gastroenteritis and also in chicken meat [5]. A phylogenetically distinct gyrovirus (GyV4) was also discovered in both human stool samples and chicken meat by 454 pyrosequencing [6]. Further 2 divergent gyroviruses, GyV5 and GyV6, were found in the stools
References
[1]
P. Biagini, M. Bendinelli, S. Hino, et al., Eds., Virus Taxonomy: Ninth Report of the International Committee on Taxonomy of Viruses, Elsevier Academic press, New York, NY, USA, 2011.
[2]
P. Biagini, “Restructuring and expansion of the family Anelloviradae. Anellviradae-Circoviradae study group,” International Committee on Taxonomy of Viruses, ICTV files and discussion, http://talk.ictvonline.org/files/proposals/taxonomy-proposals.vertebrat1/m/vert01/3911.aspx.
[3]
F. A. M. Rijsewijk, H. F. dos Santos, T. F. Teixeira et al., “Discovery of a genome of a distant relative of chicken anemia virus reveals a new member of the genus Gyrovirus,” Archives of Virology, vol. 156, no. 6, pp. 1097–1100, 2011.
[4]
V. Sauvage, J. Cheval, V. Foulongne et al., “Identification of the first human Gyrovirus, a virus related to chicken anemia virus,” Journal of Virology, vol. 85, no. 15, pp. 7948–7950, 2011.
[5]
T. G. Phan, L. Li, M. G. O’Ryan, et al., “A third gyrovirus species in human faeces,” Journal of General Virology, vol. 93, no. 6, pp. 1356–1361, 2012.
[6]
D. K. W. Chu, L. L. M. Poon, S. S. S. Chiu, et al., “Characterization of a novel gyrovirus in human stool and chicken meat,” Journal of Clinical Virology, vol. 55, no. 3, pp. 209–213, 2012.
[7]
T. G. Phan, N. P. Vo, K. Sdiri-Loulizi, et al., “Divergent gyroviruses in the feces of Tunisian children,” Journal of Virology, vol. 446, no. 1-2, pp. 346–348, 2013.
[8]
K. A. Schat, “Chicken anemia virus,” Current Topics in Microbiology and Immunology, vol. 331, pp. 151–183, 2009.
[9]
C. J. Snoeck, G. F. Komoyo, B. P. Mbee, et al., “Epidemiology of chicken anaemia virus in Central Africa Republic and Cameroon,” Journal of Virology, vol. 9, pp. 189–197, 2012.
[10]
A. M. Hegazy, F. M. Abdallah, L. K. Abd-El Samie, and A. A. Nazim, “Chicken infectious anemia virus (CIAV) in broilers and laying hens in Sharkia Province, Egypt,” Journal of American Science, vol. 6, pp. 752–758, 2010.
[11]
D. O. Oluwayelu, D. Todd, and O. D. Olaleye, “Sequence and phylogenetic analysis of chicken anaemia virus obtained from backyard and commercial chickens in Nigeria,” Onderstepoort Journal of Veterinary Research, vol. 75, no. 4, pp. 353–357, 2008.
[12]
M. F. Ducatez, A. A. Owoade, J. O. Abiola, and C. P. Muller, “Molecular epidemiology of chicken anemia virus in Nigeria,” Archives of Virology, vol. 151, no. 1, pp. 97–111, 2006.
[13]
B. O. Emikpe, D. O. Oluwayelu, O. G. Ohore, O. A. Oladele, and A. T. Oladokun, “Serological evidence of chicken anaemia virus infection in Nigerian indigenous chickens,” Onderstepoort Journal of Veterinary Research, vol. 72, no. 1, pp. 101–103, 2005.
[14]
D. O. Oluwayelu, D. Todd, N. W. Ball et al., “Isolation and preliminary characterization of chicken anemia virus from chickens in Nigeria,” Avian Diseases, vol. 49, no. 3, pp. 446–450, 2005.
[15]
A. A. Owoade, D. O. Oluwayelu, O. A. Fagbohun, W. Ammerlaan, M. N. Mulders, and C. P. Muller, “Serologic evidence of chicken infectious anemia in commercial chicken flocks in southwest Nigeria,” Avian Diseases, vol. 48, no. 1, pp. 202–205, 2004.
[16]
H. A. Hussein, M. Z. Sabry, E. A. El-Ibiary, M. El-Safty, and A. I. Abd El-Hady, “Chicken infectious anaemia virus in Egypt: molecular diagnosis by PCR and isolation of the virus from infected flocks,” Arab Journal of Biotechnology, vol. 5, no. 2, pp. 263–274, 2002.
[17]
J. D. Wicht and S. B. Maharaj, “Chicken anaemia agent in South Africa,” Veterinary Record, vol. 133, no. 6, pp. 147–148, 1993.
[18]
F. Maggi, L. Macera, D. Focosi, et al., “Human gyrovirus DNA in human blood, Italy,” Emerging Infectious Diseases, vol. 18, no. 6, pp. 956–959, 2012.
[19]
J. D. Thompson, D. G. Higgins, and T. J. Gibson, “CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice,” Nucleic Acids Research, vol. 22, no. 22, pp. 4673–4680, 1994.
[20]
K. Tamura, D. Peterson, N. Peterson, G. Stecher, M. Nei, and S. Kumar, “MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods,” Molecular Biology and Evolution, vol. 28, no. 10, pp. 2731–2739, 2011.
[21]
S. Natesan, J. M. Kataria, K. Dhama, S. Rahul, and N. Baradhwaj, “Biological and molecular characterization of chicken anaemia virus isolates of Indian origin,” Virus Research, vol. 118, no. 1-2, pp. 78–86, 2006.
[22]
Y. M. Eltahir, K. Qian, W. Jin, P. Wang, and A. Qin, “Molecular epidemiology of chicken anemia virus in commercial farms in China,” Journal of Virology, vol. 8, pp. 145–152, 2011.
[23]
S. Yamaguchi, T. Imada, N. Kaji et al., “Identification of a genetic determinant of pathogenicity in chicken anaemia virus,” Journal of General Virology, vol. 82, no. 5, pp. 1233–1238, 2001.
[24]
P. Biagini, S. Bedarida, M. Touinssi, V. Galicher, and P. de Micco, “Human gyrovirus in healthy blood donors, France,” Emerging Infectious Diseases, vol. 19, no. 6, pp. 1014–1015, 2013.
[25]
F. A. Seeliger, M. L. Brügmann, L. Krüger et al., “Porcine circovirus type 2-associated cerebellar vasculitis in postweaning multisystemic wasting syndrome (PMWS)-affected pigs,” Veterinary Pathology, vol. 44, no. 5, pp. 621–634, 2007.
[26]
S. Honma, S. Nakata, Y. Sakai, M. Tatsumi, K. Numata-Kinoshita, and S. Chiba, “Low prevalence of TT virus in the cerebrospinal fluid of viremic patients with central nervous system disorders,” Journal of Medical Virology, vol. 65, no. 2, pp. 418–422, 2001.
[27]
S. L. Smits, E. E. Zijlstra, J. J. van Hellemond, et al., “Novel cyclovirus in human cerebrospinal fluid, Malawi, 2010-2011,” Emerging Infectious Diseases, vol. 19, no. 9, pp. 1511–1513, 2013.
[28]
L. V. Tan, H. R. van Doorn, H. D. Nghia, et al., “Identification of a new cyclovirus in cerebrospinal fluid of patients with acute central nervous system infections,” MBio, vol. 4, no. 3, pp. e00231–e00213, 2013.
[29]
D. Esterhuizen, “South African poultry update: the supply and demand for broiler meat in South Africa,” http://www.thepoultrysite.com/reports/?id=1795.
[30]
S. Simionatto, C. A. da Veiga Lima-Rosa, E. Binneck, A. P. Ravazzolo, and C. W. Canal, “Characterization and phylogenetic analysis of Brazilian chicken anaemia virus,” Virus Genes, vol. 33, no. 1, pp. 5–10, 2006.
