Dietary supplementation with pomegranate juice improves isometric strength recovery after unaccustomed eccentric exercise. The purpose of this study was to determine if there is a dose response effect of pomegranate juice supplementation after eccentric exercise isometric strength recovery. Forty-five nonresistance trained, recreationally active men were assigned once-daily pomegranate juice, twice-daily pomegranate juice, or placebo supplementation. On day four of supplementation, 20？min of downhill running and 40 maximal eccentric elbow flexion repetitions were performed. Isometric knee extensor and elbow flexor strength, muscular soreness, and serum myoglobin concentrations were measured prior to exercise and 2, 24, 48, 72, and 96？h after exercise. Throughout the postexercise time period, while isometric knee extensor and elbow flexor strength were similar between once-daily and twice-daily pomegranate juice supplementation groups, isometric strength was significantly higher in pomegranate juice groups than placebo. Knee extensor soreness, elbow flexor soreness, and myoglobin increased in response to exercise but were similar between groups. It is apparent that pomegranate juice supplementation improves strength recovery in leg and arm muscles following eccentric exercise; however, no dose response effect was present. We conclude that once-daily pomegranate juice supplementation is not different from twice-daily supplementation in regards to strength recovery after eccentric exercise. 1. Introduction The performance of an acute bout of unaccustomed eccentric exercise results in immediate muscle damage and reductions in maximal strength of the exercising muscles [1]. During the 24–48-hour time period after eccentric exercise, muscular soreness peaks, after which soreness begins to return to baseline values [2]. However, maximal strength remains lower than baseline for several days [3]. This condition, delayed onset muscle soreness (DOMS), occurs in individuals who are unaccustomed to performing a high volume or intensity of eccentric contractions, but it can also occur in resistance trained individuals [4]. A goal of numerous studies has been to identify dietary supplements that accelerate recovery from a bout of intense eccentric exercise [4–20]. Antioxidant and nonsteroidal anti-inflammatory drugs (NSAIDs) have a negligible effect on strength recovery [5–13]. On the contrary, NSAID [10] and vitamin C [14, 15] supplementation have been shown to alleviate muscle soreness. Dietary supplementation with polyphenols, a class of antioxidant and
References
[1]
M. J. Gibala, J. D. MacDougall, M. A. Tarnopolsky, W. T. Stauber, and A. Elorriaga, “Changes in human skeletal muscle ultrastructure and force production after acute resistance exercise,” Journal of Applied Physiology, vol. 78, no. 2, pp. 702–708, 1995.
[2]
D. J. Newham, K. R. Mills, B. M. Quigley, and R. H. T. Edwards, “Pain and fatigue after concentric and eccentric muscle contractions,” Clinical Science, vol. 64, no. 1, pp. 55–62, 1983.
[3]
P. M. Clarkson and M. J. Hubal, “Exercise-induced muscle damage in humans,” The American Journal of Physical Medicine and Rehabilitation, vol. 81, no. 11, pp. S52–S69, 2002.
[4]
J. R. Trombold, A. S. Reinfeld, J. R. Casler, and E. F. Coyle, “The effect of pomegranate juice supplementation on strength and soreness after eccentric exercise,” Journal of Strength and Conditioning Research, vol. 25, no. 7, pp. 1782–1788, 2011.
[5]
R. A. Fielding, T. J. Manfredi, W. Ding, M. A. Fiatarone, W. J. Evans, and J. G. Cannon, “Acute phase response in exercise III. Neutrophil and IL-1β accumulation in skeletal muscle,” American Journal of Physiology—Regulatory Integrative and Comparative Physiology, vol. 265, no. 1, pp. R166–R172, 1993.
[6]
A. Childs, C. Jacobs, T. Kaminski, B. Halliwell, and C. Leeuwenburgh, “Supplementation with vitamin C and N-acetyl-cysteine increases oxidative stress in humans after an acute muscle injury induced by eccentric exercise,” Free Radical Biology and Medicine, vol. 31, no. 6, pp. 745–753, 2001.
[7]
L. J. Beaton, D. A. Allan, M. A. Tarnopolsky, P. M. Tiidus, and S. M. Phillips, “Contraction-induced muscle damage is unaffected by vitamin E supplementation,” Medicine and Science in Sports and Exercise, vol. 34, no. 5, pp. 798–805, 2002.
[8]
A. H. Goldfarb, R. J. Bloomer, and M. J. Mckenzie, “Combined antioxidant treatment effects on blood oxidative stress after eccentric exercise,” Medicine and Science in Sports and Exercise, vol. 37, no. 2, pp. 234–239, 2005.
[9]
G. L. Close, T. Ashton, T. Cable et al., “Ascorbic acid supplementation does not attenuate post-exercise muscle soreness following muscle-damaging exercise but may delay the recovery process,” British Journal of Nutrition, vol. 95, no. 5, pp. 976–981, 2006.
[10]
A. E. Donnelly, K. McCormick, R. J. Maughan, P. H. Whiting, and P. M. Clarkson, “Effects of a non-steroidal anti-inflammatory drug on delayed onset muscle soreness and indices of damage,” British Journal of Sports Medicine, vol. 22, no. 1, pp. 35–38, 1988.
[11]
P. Jakeman and S. Maxwell, “Effect of antioxidant vitamin supplementation on muscle function after eccentric exercise,” European Journal of Applied Physiology and Occupational Physiology, vol. 67, no. 5, pp. 426–430, 1993.
[12]
S. R. J. Maxwell, P. Jakeman, H. Thomason, C. Leguen, and G. H. G. Thorpe, “Changes in plasma antioxidant status during eccentric exercise and the effect of vitamin supplementation,” Free Radical Research Communications, vol. 19, no. 3, pp. 191–202, 1993.
[13]
D. A. J. Connolly, C. Lauzon, J. Agnew, M. Dunn, and B. Reed, “The effects of vitamin C supplementation on symptoms of delayed onset muscle soreness,” Journal of Sports Medicine and Physical Fitness, vol. 46, no. 3, pp. 462–467, 2006.
[14]
M. Kaminski and R. Boal, “An effect of ascorbic acid on delayed onset muscle soreness,” Pain, vol. 50, no. 3, pp. 317–321, 1992.
[15]
S. C. Bryer and A. H. Goldfarb, “Effect of high dose vitamin C supplementation on muscle soreness, damage, function, and oxidative stress to eccentric exercise,” International Journal of Sport Nutrition and Exercise Metabolism, vol. 16, no. 3, pp. 270–280, 2006.
[16]
J. L. Bowtell, D. P. Sumners, A. Dyer, P. Fox, and K. N. Mileva, “Montmorency cherry juice reduces muscle damage caused by intensive strength exercise,” Medicine and Science in Sports and Exercise, vol. 43, no. 8, pp. 1544–1551, 2011.
[17]
D. A. J. Connolly, M. P. McHugh, and O. I. Padilla-Zakour, “Efficacy of a tart cherry juice blend in preventing the symptoms of muscle damage,” British Journal of Sports Medicine, vol. 40, no. 8, pp. 679–683, 2006.
[18]
G. Howatson, M. P. McHugh, J. A. Hill et al., “Influence of tart cherry juice on indices of recovery following marathon running,” Scandinavian Journal of Medicine and Science in Sports, vol. 20, no. 6, pp. 843–852, 2010.
[19]
J. R. Trombold, J. N. Barnes, L. Critchley, and E. F. Coyle, “Ellagitannin consumption improves strength recovery 2-3 d after eccentric exercise,” Medicine and Science in Sports and Exercise, vol. 42, no. 3, pp. 493–498, 2010.
[20]
K. S. Kuehl, E. T. Perrier, D. L. Elliot, and J. C. Chesnutt, “Efficacy of tart cherry juice in reducing muscle pain during running: a randomized controlled trial,” Journal of the International Society of Sports Nutrition, vol. 7, article 17, 2010.
[21]
C. Manach, G. Williamson, C. Morand, A. Scalbert, and C. Remesy, “Bioavailability and bioefficacy of polyphenols in humans. I. Review of 97 bioavailability studies,” The American Journal of Clinical Nutrition, vol. 81, pp. 230S–242S, 2005.
[22]
M. I. Kingsley, L. P. Kilduff, J. Mceneny, R. E. Dietzig, and D. Benton, “Phosphatidylserine supplementation and recovery following downhill running,” Medicine and Science in Sports and Exercise, vol. 38, no. 9, pp. 1617–1625, 2006.
[23]
J. M. Peake, K. Suzuki, G. Wilson et al., “Exercise-induced muscle damage, plasma cytokines, and markers of neutrophil activation,” Medicine and Science in Sports and Exercise, vol. 37, no. 5, pp. 737–745, 2005.
[24]
P. M. Clarkson, W. C. Byrnes, K. M. McCormick, L. P. Turcotte, and J. S. White, “Muscle soreness and serum creatine kinase activity following isometric, eccentric, and concentric exercise,” International Journal of Sports Medicine, vol. 7, no. 3, pp. 152–155, 1986.
[25]
G. L. Warren, D. A. Lowe, and R. B. Armstrong, “Measurement tools used in the study of eccentric contraction-induced injury,” Sports Medicine, vol. 27, no. 1, pp. 43–59, 1999.
[26]
F. X. Pizza, T. J. Koh, S. J. McGregor, and S. V. Brooks, “Muscle inflammatory cells after passive stretches, isometric contractions, and lengthening contractions,” Journal of Applied Physiology, vol. 92, no. 5, pp. 1873–1878, 2002.
