Malaria-associated hemolysis is associated with mortality in adult patients. It has been speculated that oxidative stress and inflammation induced by malaria parasite are involved in its pathophysiology. Hence, we aimed to investigate the antihemolytic effect of green tea, safflower, and mulberry extracts against Plasmodium berghei infection. Aqueous crude extracts of these plants were prepared using hot water method and used for oral treatment in mice. Groups of ICR mice were infected with 6 × 106 infected red blood cells of P. berghei ANKA by intraperitoneal injection and given the extracts (500, 1500, and 3000？mg/kg) twice a day for 4 consecutive days. To assess hemolysis, hematocrit levels were then evaluated. Malaria infection resulted in hemolysis. However, antihemolytic effects were observed in infected mice treated with these extracts at dose-dependent manners. In conclusion, aqueous crude extracts of green tea, safflower, and mulberry exerted antihemolysis induced by malaria infection. These plants may work as potential source in the development of variety of herbal formulations for malarial treatment. 1. Introduction Malaria is an enormous public health problem worldwide, especially tropical and subtropical area, and kills 730,000 people annually mostly children residing in Africa. It is caused by the parasite Plasmodium and transmitted by the bite of Anopheles mosquito [1]. Malaria-associated acute hemolysis, one of the major life-threatening well-known causes of death in P. falciparum and P. vivax, occurs between 1 and 4% of hospitalized patients with a mortality that can be up to 45% [2, 3]. The pathogenesis of malarial-associated acute hemolysis has suggested involvement of cytoadherence of infected red blood cell (RBC) and inflammatory response as well as oxidative stress through generation of reactive oxygen intermediates by host cells [4, 5]. Moreover, parasite invasion and subsequent RBC rupture also contributed to pathogenesis of hemolysis. This has prompted research towards the discovery and development of new, safe, and affordable antihemolysis drugs during malaria infection. In this respect, medicinal plants are potential targets for research. Recently, interest in green tea (Camellia sinensis), safflower (Carthamus tinctorius), and mulberry (Morus alba) as promising agents for the prevention or reduction of risk for many human diseases involving oxidative stress has increased. They are popular beverage worldwide which contain large amounts of polyphenols and flavonoids. The possible beneficial effects of these tea extracts in the
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