类胡萝卜素对前脂肪细胞3T3-L1增殖和分化的影响
Effects of Carotenoids on 3T3-L1 Preadipocyte Viability and Differentiation

DOI: 10.12677/BIPHY.2015.32005, PP. 49-57

Keywords: 类胡萝卜素，3T3-L1前脂肪细胞，分化，PPARγ
Carotenoids, 3T3-L1 Preadipocytes, Differentiation, PPARγ

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Abstract:

本文通过评估胭脂树橙、β-胡萝卜素和番茄红素对3T3-L1前脂肪细胞活力和分化的影响，探究其可能作用机制。以四甲基偶氮唑盐(MTT)法检测细胞活力，用油红O染色和染色比色法分析脂肪细胞的分化程度，West blotting检测过氧化物酶体增殖物激活受体gamma (PPARγ)的表达水平。结果表明，胭脂树橙、β-胡萝卜素和番茄红素明显降低3T3-L1细胞活力，抑制其分化，下调PPARγ的表达水平。β-胡萝卜素比胭脂树橙和番茄红素表现出更强的抑制作用。结果提示胭脂树橙、β-胡萝卜素和番茄红素经由PPARγ下调抑制3T3-L1细胞分化。
The aim of the study was to assess the suppressive effects of bixin, β-carotene and lycopene on the differentiation of 3T3-L1 preadipocytes. The viability of 3T3-L1 cells were tested by MTT and their differentiation was evaluated by Oil red O staining method. The expression levels of peroxisome proliferator-activated receptor gamma (PPARγ) were measured by West blotting. The results showed that bixin, β-carotene and lycopene significantly reduced the viability and suppressed the differentiation of 3T3-L1 preadipocytes, respectively. β-Carotene had stronger suppressive effects than bixin and lycopene on adipocyte differentiation in 3T3-L1 cells. Bixin, β-carotene and lycopene down-regulated PPARγ expression. These results suggest that bixin, β-carotene and lycopene inhibit the adipocyte differentiation of 3T3-L1 cells through down-regulation of PPARγ.

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