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冰川冻土  2015 

青藏高原土壤中一株低温原油降解菌的分离鉴定及其原油降解特性

DOI: 10.7522/j.issn.1000-0240.2015.0060, PP. 528-537

Keywords: 原油降解菌,红球菌属,alkB,almA,青藏高原

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Abstract:

从青藏高原班戈桥地区土壤中分离到一株能利用原油为碳源生长的细菌(BGQ-6).通过16SrRNA基因序列比对及BiologGENⅢ鉴定板确定该菌株为Rhodococcusqingshengii.将生长至对数期的菌株接入MM培养基,10℃、150rpm条件下培养15d后,通过GC法检测到该菌对原油的总降解率为74.14%,且对直链烷烃、支链烷烃、环烷烃和芳香烃等60种烃类有较高的降解率.通过特异性基因扩增检测到该菌株基因组中具有4个alkB和1个almA两种烷烃羟化酶基因.

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