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草业科学  2015 

蒺藜苜蓿EMS诱变突变体库的构建及突变体表型的分析

DOI: 10.11829\j.issn.1001-0629.2014-0034, PP. 71-77

Keywords: 蒺藜苜蓿,甲基磺酸乙酯,诱变

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Abstract:

?突变体是进行功能基因组学研究的重要材料,大量基因已经从各种突变体中得到克隆。本研究利用甲基磺酸乙酯(EMS)诱变蒺藜苜蓿(Medicagotruncatula)品种A17种子,鉴定和统计其后代突变型和突变频率,分别获得2085份M1代和388份M2代突变材料,M2代突变材料中叶色突变性状包括叶斑突变、叶深绿、杂色叶、叶红紫、叶黄萎和白化苗,占群体的5.37%;叶型突变性状包括皱叶、窄叶、卷曲和多叶等,占3.12%;株型突变性状包括高秆、矮化、匍匐、半匍匐、多分枝和少分枝等,占9.11%;开花期突变性状包括早花、晚花等,占1.01%;总的表型突变频率为18.61%。本研究初步构建的蒺藜苜蓿A17EMS的突变材料一方面创造了蒺藜苜蓿新的种质资源,另一方面可应用于蒺藜苜蓿功能基因组的研究。

References

[1]  The Arabidopsis Genome Initiative.Analysis of the genome sequence of the flowering plant Arabidopsis thaliana.Nature,2000,408(6814):796-815.
[2]  Sasaki T,Matsumoto T,Yamamoto K,Sakata K,Baba T,Katayose Y,Wu J,Niimura Y,Cheng Z,Nagamura Y,Antonio B A,Kanamori H,Hosokawa S,Masukawa M,Arikawa K,Chiden Y,Hayashi M,Okamoto M,Ando T,Aoki H,Arita K,Hamada M,Harada C,Hijishita S,Honda M,Ichikawa Y,Idonuma A,Iijima M,Ikeda M,Ikeno M I.The genome sequence and structure of rice chromosome 1.Nature,2002,420(6913):312-316.
[3]  郭建秋,雷全奎,杨小兰,马雯,张向召.植物突变体库的构建及突变体检测研究进展.河南农业科学,2010,6(6):150-155.
[4]  Ma L C,Wang Y R,Liu W X,Liu Z P.Expression analysis of seed-specific genes in four angiosperm species with an emphasis on the unconserved expression patterns of homologous genes.Seed Science Research,2013,23(4):223-231.
[5]  顾佳清,张智奇,周音,奚银兴,张建军,胡筱荷,郑雷英,洪祥宾,杨军,罗达,朱旭东.EMS诱导水稻中花11突变体的筛选和鉴定.上海农业学报,2005,21(1):7-11.
[6]  McCallum C M,Comai L,Greene E A,Henikoff S.Targeted screening for induced mutations.Nature Biotechnology,2000,18(4):455-457.
[7]  库来宝.EMS玉米花粉诱变及根系突变体筛选.北京:中国农业大学,2007.
[8]  吴文波.EMS诱导甘蓝型油菜耐旱突变体的筛选.重庆:西南大学,2011.
[9]  张娜,杨希文,任长忠,胡银岗.白燕2号EMS突变体的形态鉴定与遗传变异分析.麦类作物学报,2011,31(3):421-426.
[10]  曹嵩晓.广藿香无菌苗的秋水仙素和EMS诱变及突变体植株遗传多样性的ISSR分析.海口:海南大学,2011.
[11]  张晓勤,薛大伟,周伟辉,邬飞波,张国平.用甲基磺酸乙酯(EMS)诱变的大麦浙农大3号突变体的筛选和鉴定.浙江大学学报(农业与生命科学版),2011,37(2):169-174.
[12]  刘志斋.玉米EMS处理所得突变系的诱变效应研究.重庆:西南农业大学,2005.
[13]  关艳龙,李婉莎,殷奎德,杨永平,胡向阳.激活标签法构建拟南芥突变体库及其表型分析.云南植物研究,2010,32(1):53-59.
[14]  Till B J,Reynolds S H,Weil C,Springer N,Burtner C,Young K,Bowers E,Codomo C A,Enns L C,Odden A R,Greene E A,Comai L,Henikoff S.Discovery of in duced point mutations in maize genes by TILLING.BMC Plant Biology,2004,4:12.
[15]  Caldwell D G,McCallum N,Shaw P,Caldwell D G,Shaw P,Muehlbauer G J,Marshall D F,Waugh R.A structured mutant population for forward and reverse genetics in barley (Hordeum vulgare L.) .The Plant Journal,2004,40(1):143-150.
[16]  Slade A J,Fuerstenberg S I,Loeffler D,Steine M N,Facciotti D.A reverse genetic,nontransgenic approach to wheat crop improvement by TILLING.Nature Biotechnology,2004,23(1):75-81.
[17]  Chiu W H,Chandler J,Cnops G,Lijsebettens M V,Werr W.Mutations in the TORNADO2 gene affect cellular decisions in the peripheral zone of the shoot apical meristem of Arabidopsis thaliana.Plant Molecular Biology,2007,63(6):731-744.
[18]  于秀普,杜连恩,魏玉昌,可福存.大豆新品种冀豆8号的选育.中国油料作物学报,1994,16(4):58-59.
[19]  Maluszynski M,Ahloowalia B S,Sigurbjrnsson B.Application of in vivo and in vitro mutation techniques for crop improvement.Plant Sciences,1995,3(11):303-315.
[20]  陈爱民,连瑞丽,孙杰,王彦章.豆科模式植物——蒺藜苜蓿.植物生理学通讯,2006,42(5):997-1003.
[21]  雷艳芳.蒺藜苜蓿遗传图谱的构建及种子产量相关性状的遗传分析.兰州:甘肃农业大学,2009.
[22]  Penmetsa R V,Cook D R.Production and characterization of diverse developmental mutants of Medicago truncatula.Plant Physiology,2000,123(4):1387-1397.
[23]  Tadege M,Wen J,He J,Tu H,Kwak Y,Eschstruth A,Cayrel A,Endre G,Zhao P X,Chabaud M,Ratet P,Mysore K S.Large-scale insertional mutagenesis using the Tnt1 retrotransposon in the model legume Medicago truncatula.The Plant Journal,2008,54(2):335-347.
[24]  韦祖生,李开绵.作物诱变育种及突变体鉴定与筛选研究进展.江西农业学报,2007,19(10):38-41.
[25]  朱正歌,肖晗,傅亚萍,胡国成,于永红,斯华敏,张景六,孙宗修.水稻转座子突变体库的构建级突变类型的遗传分析.生物工程学报,2001,17(3):288-292.
[26]  刘治先.玉米育种新技术.玉米科学,1995,3(4):12-15.
[27]  张凤启,黄永娟,杨甜甜,陈健美,管荣展,张红生.EMS诱变甘蓝型油菜M2代群体的表型突变研究.植物遗传资源学报,2010,11(6):760-765.
[28]  余凤群,金明源,肖才升,王蕾.甘蓝型油菜DH群体几个数量性状的遗传分析.中国农业科学,1998,31(3):44-48.
[29]  杨青川,刘志鹏,呼天明,胡晓艳.DNA分子标记技术在苜蓿研究中的应用.中国农业科技导报,2004,6(2):30-34.
[30]  李卫华,胡志伟,褚洪雷,薛芳,张东海.EMS对小麦产量性状和农艺性状诱变效应的研究.种子,2011,30(2):41-44.
[31]  魏玉昌,杜连恩,于秀普,吴鸣岐.甲基磺酸乙酯诱发大豆合子突变效果的研究.中国油料作物学报,1999,21(3):34-36.
[32]  汪念.甘蓝型油菜EMS突变体库的构建及TILLING、Eco-TILLING技术的应用研究.武汉:华中农业大学,2009.
[33]  Wang N,Wang Y J,Tian F,King G J,Zhang C Y,Long Y,Shi L,Meng J L.A functional genomics resource for Brassica napus:Development of an EMS mutagenized population and discovery of FAE1 point mutations by TILLING .New Phytologist,2008,180(4):751-765.
[34]  Greene E A,Codomo C A,Taylor N E,Henikoff J G,Till B J,Reynolds S H,Enns L C,Chris Burtner C,Johnson J E,Odden A R,Comai L,Henikoff S.Spectrum of chemically induced mutations from a large-scale reverse-genetic screen in Arabidopsis thaliana.Genetics,2003,164(2):731-740.
[35]  Kuraparthy V,Sood S,Dhaliwal H S,Chhuneja P,Gill B S.Identification and mapping of a tiller inhibition gene (tin3) in wheat.Theoretical and Applied Genetics,2007,114(2):285-294.
[36]  祝丽英,池书敏.刘志增,王静华.甲基磺酸乙酯在创造玉米新种质中的应用.玉米科学,2001,9(3):14-17.

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