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核农学报  2015 

水稻条白叶和白穗突变基因St-wp的遗传分析与精细定位

DOI: 10.11869/j.issn.100-8551.2015.07.1246, PP. 1246-1252

Keywords: 突变体,条白叶,白穗,遗传分析,水稻

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Abstract:

为研究叶绿体的差异性发育,本文通过60Co-γ诱变籼稻93-11筛选获得条白叶片和白穗突变体。经遗传分析表明,该突变性状受单隐性核基因控制,暂命名为St-wp(stripewhiteleafandwhitepanicle)。突变基因St-wp被精细定位于第6染色体标记dCAMPs620和InDel620之间,物理距离为9.2kb,此区间内包含3个候选基因。测序对比发现,突变体基因组的核糖核苷二磷酸还原酶小亚基(ribonucleoside-diphosphatereductasesmallchain)编码区第308碱基由A变为T,导致谷氨酸突变为缬氨酸。该基因与已报道的水稻白条纹叶基因St1和Gws等位,但突变体st1和gws均未表现出白穗性状。本研究可为叶绿体组织差异性发育的深入研究提供依据。

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