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核农学报  2015 

茶树茶氨酸代谢相关基因表达组织特异性分析

DOI: 10.11869/j.issn.100-8551.2015.07.1285, PP. 1285-1291

Keywords: 茶树,茶氨酸代谢,荧光定量PCR,组织特异性

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Abstract:

为了研究茶树中茶氨酸代谢相关基因的组织与表达特性,本研究利用实时荧光定量PCR检测方法探索了茶树(CamelliaSinensis)茶氨酸代谢途径中相关基因:茶氨酸合成酶(TS)、谷氨酰胺合成酶(GS)、谷氨酰胺α-酮戊二酸氨基转移酶(GOGAT)、谷氨酸脱氢酶(GDH)以及与氮素吸收和转化密切相关的亚硝酸还原酶(NiR)、精氨酸脱羧酶(ADC),在不同组织器官及不同发育时期的表达情况。结果表明,TS基因家族成员在茶树的不同器官中均有表达,且差异明显,其中TS1在根中表达量最高,TS2在花中表达量最高,而TS3在单芽和侧根中的表达量均相对较高;而GS、GOGAT基因均明显表现为叶部含量高于根部,说明其主要参与了叶部光呼吸过程释放氨的再同化过程;对于GDH、NiR、ADC等主要参与根部铵态氮吸收与转化的基因则表现为根部含量明显超过叶部。表明茶氨酸合成途径同时受到根部与叶部氮代谢的影响,同时茶氨酸合成酶基因家族成员的表达呈现出明显的组织特异性。本研究有助于从分子水平上分析与认识影响茶树茶氨酸代谢途径相关基因的表达与调控模式,为今后茶树品质遗传改良提供理论基础。

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