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核农学报  2015 

cspB异源表达对提高转基因烟草植株耐高温能力的影响

DOI: 10.11869/j.issn.100-8551.2015.06.1068, PP. 1068-1073

Keywords: cspB基因,转基因植株,高温抗性,烟草

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Abstract:

为了解枯草芽孢杆菌cspB基因在植物中异源表达对植物应对逆境的影响,将cspB基因插入到pBI121的35S启动子下游取代原有的gusA基因,该基因的植物表达载体被命名为pBI121-cspB,通过农杆菌介导的转化法转化烟草,通过筛选培养获得转基因植株。RT-PCR分析表明,转基因烟草株系中均有cspB基因表达,其中TN012株系中表达量较大。转基因植株TN012和非转基因对照植株经38℃高温处理一周,再在25℃条件下恢复生长,经过7d的恢复生长,TN012的生物量明显高于对照植株。高温处理前,TN012与对照植株光合速率无明显差异,高温处理后TN012的光合速率显著高于对照植株的。TN012细胞中的可溶性蛋白在高温处理后明显上升,而对照植株的则明显下降。结果表明cspB基因植物异源表达提高植物对高温的抗性,cspB基因可以作为用于耐高温作物育种的基因资源。

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