全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...
核农学报  2015 

花生FUSCA3基因的克隆与表达分析

DOI: 10.11869/j.issn.100-8551.2015.06.1044, PP. 1044-1051

Keywords: 花生,FUSCA3基因,克隆,系统发育分析,荧光定量PCR

Full-Text   Cite this paper   Add to My Lib

Abstract:

为了挖掘花生品质性状相关功能基因,本研究以花生品种花育17号为试材,根据cDNA文库中已知的FUSCA3基因全长序列设计引物,通过RT-PCR克隆到该基因,命名为AhFUSCA3。AhFUSCA3全长为1532bp,开放阅读框ORF为1134bp,对应的基因组序列3892bp,由6个外显子和5个内含子组成,内含子剪接方式符合GT-AG剪接规则。根据编码区预测AhFUSCA3编码一条378个氨基酸组成的多肽,预测分子量为42.3kD,等电点为5.58。预测该基因编码的蛋白含有B3保守结构域,可能定位于细胞核中。蛋白序列比对和进化树分析表明,花生与大豆(Glycinemax)、菜豆(Phaseolusvulgaris)、鹰嘴豆(Cicerarietinum)和苜蓿(Medicagotruncatula)等豆科植物中的FUSCA3序列相似性最高,亲缘关系最近。荧光定量PCR结果显示,AhFUSCA3基因表达量在花生荚果、种子发育的过程中呈现先增后降的趋势,推测AhFUSCA3基因在花生胚胎形成和发育过程中发挥重要作用。本研究为阐明花生胚胎发育过程和油脂代谢机制提供了理论基础,为花生品质改良育种提供了新的基因资源。

References

[1]  Müller A J. Embryonentest zum Nachweis rezessiver Letalfaktoren bei Arabidopsis thaliana [J]. Biologisches Zentralblatt, 1963, 82: 133-163
[2]  Luerssen H, Kirik V, Herrmann P and Misera S. FUSCA3 encodes a protein with a conserved VP1/AB13-like B3 domain which is of functional importance for the regulation of seed maturation in Arabidopsis thaliana [J]. The Plant Journal, 1998, 15(6): 755-764
[3]  Fankhauser C, Chory J. Light control of plant development [J]. Annual Review of Cell and Developmental Biology, 1997, 13(1): 203-229
[4]  Lotan T, Ohto M, Yee M K, West M A, Lo R, Kwong R W, Yamagishi K, Fischer R L, Goldberg R B and Harada J J. Arabidopsis LEAFY COTYLEOON1 is sufficient to induce embryo development in vegetative cells [J]. Cell, 1998, 93(7): 1195-1205
[5]  Stone S L, Kwong L W, Yee K M, Pelletier J, Lepiniec L, Fischer R L, Goldberg R B and Harada J J. LEAFY COTYLEDON2 encodes a B3 domain transcription factor that induces embryo development [J]. Proceedings of the National Academy of the Sciences of the United States of America, 2001, 98(20): 11806-11811
[6]  Kroj T, Savino G, Valon C, Giraudat J, Parcy F. Regulation of storage protein gene expression in Arabidopsis [J]. Development, 2003, 130(24): 6065-6073
[7]  Monke G, Altschmied L, Tewes A, Reidt W, Mock H P, Baumlein H and Conrad U. Seed-specific transcription factors ABI3 and FUS3: molecular interaction with DNA [J]. Planta, 2004, 219(1): 158-166
[8]  Baumlein H, Misera S, Luerssen H, Kolle K, Horsemann C, Wobus U and Muller A J. The FUS3 gene of Arabidopsis thaliana is a regulator of gene expression during late embryogenesis [J]. The Plant Journal, 1994, 6(3): 379-387
[9]  Parcy F, Valon C, Kohara A. The ABSCISIC ACIDINSENSIT-IVE3, FUSCA3, and LEAFY COTYLEDON1 loci act in concert to control multiple aspects of Arabidopsis seed development [J]. Plant Cell, 1997, 9(8): 1265-1277
[10]  Nambara E, Hayama R, Tsuchiya Y, Nishimura M, Kawaide H, Kamiya Y, Naito S. The role of ABI3 and FUS3 loci in Arabidopsis thaliana on phase transition from late embryo development to germination [J]. Developmental Biology, 2000, 220(2): 412-423
[11]  Ikeda-Iwai, Schiavo F. Stress-induced somatic embryogenesis in vegetative tissues of Arabidopsis thaliana [J].The Plant Journal, 2003, 34(1):107-114
[12]  夏晗, 王兴军, 李孟军, 肖寒. 利用基因工程改良植物脂肪酸和提高植物含油量的研究进展[J]. 生物工程学报, 2010, 26(6): 735-743
[13]  殷冬梅, 宋佳静, 张幸果, 李贺敏, 王允, 崔党群. 花生种子在不同发育时期的显微结构分析[J]. 核农学报, 2013, 27(3): 344-349
[14]  Tamura K, Dudley J, Nei M, Kumar S. MEGA4: Molecular evolutionary genetics analysis (MEGA) software version 4.0 [J]. Molecular Biology and Evolution, 2007, 24(8):1596-1599
[15]  Chi X Y, Hu R B, Yang Q L, Zhang X W, Pan L J, Chen N, Chen M N, Yang Z, Wang T, He Y N, Yu S L. Validation of reference genes for gene expression studies in peanut by quantitative real-time RT-PCR [J]. Molecular Genetics and Genomics, 2011, 287(2): 167-176
[16]  Pan L J, Yang Q L, Chi X Y, Chen M N, Yang Z, Chen N, Wang T, Wang M, He Y N, Yu S L. Functional analysis of the phosphoenolpyruvate carboxylase on the lipid accumulation of peanut (Arachis hypogaea L.) seeds [J]. Journal of Integrative Agriculture, 2013, 13(1): 36-44
[17]  Keith K A, Kraml M, Dengler N G, McCourt P. fusca3: a heterchronic mutation affecting late development in Arabidopsis[J]. Plant Cell, 1994, 6(5): 589-600
[18]  Meinke D, Franzmann L H, Nickle T C, Yeung E C. Leafy cotyledon mutants of Arabidopsis[J]. Plant Cell, 1994, 6(8): 1049-1064
[19]  Meinke D W. Perspectives on genetic analysis of plant embryogenesis [J]. Plant Cell, 1991, 3(9): 857-866
[20]  West M and Harada J J. Embryogenesis in higher plants: An overview [J]. Plant Cell, 1993, 5(10): 1361-1369
[21]  Parcy F, Valon C, Raynal M, Gaubiercomella P, Delseny M, Giraudat J. Regulation of Gene-Expression Programs During Arabidopsis Seed Development- Roles of the abi3 Locus and of Endogenous Abscisic-Acid [J]. Plant Cell, 1994, 6(11): 1567-1582
[22]  Lumba S, Tsuchiya Y, Delmas F, Hezky J, Provart N J, Lu Q S, McCourt P, Gazzarrini S. The embryonic leaf identity gene FUSCA3 regulates vegetative phase transitions by negatively modulating ethylene-regulated gene expression in Arabidopsis [J]. BMC Biology, 2012, 10:8.doi:10.1186/1741-7007-10-8
[23]  Chiu R S, Nahal H, Provart N J and Gazzarrini S. The role of the Arabidopsis FUSCA3 transcription factor during inhibition of seed germination at high temperature [J]. BMC Plant Biology, 2012, 12:15.doi:10.1186/1471-2229-12-15
[24]  Curaba J, Moritz T, Blervaque R, Parcy F, Raz V, Herzog M, Vachon G. AtGA3ox2, a key gene responsible for bioactive gibberellin biosynthesis, is regulated during embryogenesis by LEAFY COTYLEDON2 and FUSCA3 in Arabidopsis [J]. Plant Physiology, 2004, 136(3): 3660-3669
[25]  Mu J Y, Tan H L, Zheng Q, Fu F Y, Liang Y, Zhang J, Yang X H, Wang T, Chong K, Wang X J, Zuo J. LEAFY COTYLEDON1 is a key regulator of fatty acid biosynthesis in Arabidopsis [J]. Plant Physiology, 2008, 148(10): 1042-1054
[26]  Wang H, Guo J, Lambert K, Lin Y. Developmental control of Arabidopsis seed oil biosynthesis [J]. Planta, 2007, 226(3):773-783
[27]  Santos Mendoza M, Dubreucq B, Miquel M, Caboche M, Lepiniec L. LEAFY COTYLEDON 2 activation is sufficient to trigger the accumulation of oil and seed specific mRNAs in Arabidopsis leaves [J]. FEBS Letters, 2005, 579(21): 4666-4670

Full-Text

Contact Us

[email protected]

QQ:3279437679

WhatsApp +8615387084133