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菌物学报  2015 

外源一氧化氮提高白灵侧耳菌丝耐热性生化途径分析

DOI: 10.13346/j.mycosystema.150038, PP. 632-639

Keywords: 白灵侧耳,NO,高温胁迫,抗氧化酶,耐热性

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Abstract:

为了探索外源一氧化氮(NO)提高食用菌菌丝体耐热性的生化途径,以白灵侧耳Pleurotuseryngiivar.tuoliensis菌株CCMSSC00489为材料,通过测定高温胁迫下外源添加硝普钠(sodiumnitroprusside,SNP,NO供体)后,菌丝体内超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽还原酶(GR)和过氧化物酶(POD)等4个抗氧化酶活性的变化,研究外源NO在高温胁迫响应中对抗氧化酶的影响。试验表明,高温胁迫致使菌丝体内TBARS含量升高,膜脂过氧化加剧。在正常温度培养(CK)下,外源添加SNP无显著缓解膜脂过氧化的效果,而高温胁迫条件下缓解效果显著,高温胁迫6h和12h,TBARS含量较对照(未添加)分别下降31.5%和25%。研究表明,抗氧化酶类对外源NO的响应不同。在有外源添加SNP的高温胁迫条件下,菌丝体内的SOD、CAT和GR活性随处理时间的延长而显著增强,在处理72h达到最高,分别是对照(0h)的1.73、7.29和4.95倍。其中CAT是高温胁迫响应的主要抗氧化酶类,其活力可以mmol/L·min-1·mg-1ofprotein计量,而其他种类的活力均仅以μmol/L·min-1·mg-1ofprotein计量。在试验条件下,这些抗氧化酶类活性的提高与TBARS含量的降低相呼应,表明外源NO通过提高SOD、CAT、GR的活性降低高温胁迫下的活性氧水平,缓解其氧化损伤,提高菌丝体耐热性。POD活性在外源添加SNP的高温胁迫条件下显著降低。

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