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菌物学报  2015 

冠突散囊菌转录因子stf1基因的克隆及其表达分析

DOI: 10.13346/j.mycosystema.140114, PP. 91-97

Keywords: 冠突散囊菌,转录因子,基因表达

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Abstract:

利用RACE技术获得了冠突散囊菌stf1基因的全长DNA序列。该序列全长3029bp,开放阅读框长度为2664bp,从114–2908bp,在253bp处含有一个131bp的内含子,预测编码887个氨基酸。同源分析结果表明该基因与Snd1/p100转录因子同源。应用相对定量SYBRGreenI荧光定量PCR技术对stf1基因在不同发育时期的表达量的差异进行了检测。结果表明,这个基因在子囊孢子时期的表达量最高,在分生孢子时期的表达量最低,相比子囊孢子时期下降了1倍。为深入研究冠突散囊菌的产孢调控机制奠定了一定的基础。

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