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菌物学报  2015 

药用拟层孔菌石油醚提取物的GC-MS分析及各相提取物生物活性

DOI: DOI:10.13346/j.mycosystema.140142, PP. 482-489

Keywords: 药用拟层孔菌,抗肿瘤,抗氧化

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Abstract:

通过形态学和分子生物学分析,实验菌株为药用拟层孔菌。采用不同有机溶剂提取药用拟层孔菌子实体,分别获得石油醚、氯仿、乙酸乙酯和甲醇提取物。采用GC-MS方法,分析药用拟层孔菌石油醚提取物中的化学成分,同时对不同有机溶剂提取物的生物活性进行检测。结果表明:药用拟层孔菌石油醚提取物中共鉴定出46个化合物。体外抑制肿瘤细胞活性实验表明,在100μg/mL时,不同有机溶剂提取物都表现出抑制肿瘤细胞活性。其中,石油醚提取物在50μg/mL时,其对NCI-H460和SGC-7901肿瘤细胞的抑制率分别为99.03%和82.57%。甲醇提取物在8mg/mL时,DPPH自由基清除率达93.35%。

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