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菌物学报  2015 

谢瓦氏曲霉间型变种esdC基因的功能分析

DOI: DOI:10.13346/j.mycosystema.130237, PP. 227-234

Keywords: 谢瓦氏曲霉间型变种,esdC基因,基因敲除,功能分析

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Abstract:

为了研究谢瓦氏曲霉间型变种esdC基因的功能,构建该基因的敲除载体,通过农杆菌介导转化及筛选获得敲除突变体,并对突变体的形态进行了初步分析。生物学特性显示:△esdC突变子的子囊结构与野生型相似,子囊孢子均为球形或近球形,无性发育也与野生型无显著差异。但是,在MYA培养基上培养14d后,△esdC突变体菌落的渗出液相对野生型较少,且无皱褶,菌落表面干燥。这些结果表明esdC基因的缺失对孢子结构的影响不显著,为谢瓦氏曲霉间型变种有性调控机制的研究奠定了技术基础。

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