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菌物学报  2015 

绒泡菌目黏菌的ITS1-5.8S-ITS2二级结构的比较分析

DOI: DOI:10.13346/j.mycosystema.130224, PP. 235-245

Keywords: 绒泡菌目,ITS,RNA二级结构,模型,互补性碱基变化,分子进化

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Abstract:

为探讨核糖体DNA转录间隔区(rDNAITS)的RNA二级结构在黏菌系统发育研究中的作用,以黏菌ITS通用引物PHYS4和PHYS5对绒泡菌目5属8种黏菌的rDNAITS进行扩增和测序,利用RNAstructure构建了ITS区的RNA二级结构模型。结果表明:ITS1在绒泡菌目黏菌中不能形成一个紧实的结构,但大部分物种都具有一段稳定的螺旋结构,可能对rRNA的成熟具有作用;5.8SrRNA的二级结构相似,由4个螺旋组成,主要为两种类型;基于5.8SrRNA和28SrRNA相互作用构建的ITS2的二级结构模型显示,它由一个封闭的多分支环和至少4个主要的螺旋组成,其中螺旋IV结构相对比较保守。由于ITS区的二级结构相比核苷酸序列更加保守,因此深入地分析其二级结构有助于认识其结构与进化的关系。

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