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棉花学报  2015 

棉花黄萎病菌VdSge1基因的敲除与功能分析

DOI: 10.11963/issn.1002-7807.201504007, PP. 346-353

Keywords: 棉花,大丽轮枝菌,VdSge1基因,基因敲除,基因功能

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Abstract:

为了明确棉花黄萎病菌大丽轮枝菌(VerticilliumdahliaeKleb.)的VdSge1基因功能,本研究利用基因同源重组原理,通过构建敲除载体,对棉花黄萎病菌落叶型强致病力菌株V592的VdSge1基因进行了敲除,获得了4个目标基因敲除的突变体;以野生型菌株V592为对照,通过对敲除突变体生物学性状和致病性测定,结果表明,突变体的菌落生长速度、产孢量明显高于野生型,并且丧失对棉花的致病性。利用qRT-PCR对其他基因在突变体中的表达情况进行分析,结果表明,VdSge1基因敲除后,VMK1、VGB和VdGARP1的表达量随之下降,而VDH1和PevD1的表达却明显上升。由此说明,VdSge1基因与大丽轮枝菌的菌落生长速度、产孢量及致病性密切相关,并且可以影响其他一些基因的表达。

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