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棉花学报  2015 

半野生棉棉仁含油量与SSR标记的关联分析

DOI: 10.11963/issn.1002-7807.201502002, PP. 104-110

Keywords: 半野生棉,含油量,关联分析

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Abstract:

为探究半野生棉群体材料的遗传变异和群体结构，挖掘与含油量相关的标记位点，利用215对SSR引物，对89份半野生棉自然群体进行遗传多样性、群体结构和亲缘关系分析，并进行棉仁含油量性状与标记的关联分析。结果表明，位点多态信息含量（PIC）为0.0222～0.7390，平均为0.3452；基因型多样性（H′）介于0.0616～1.4412，平均为0.5614。群体结构分析将89份材料分为2个亚群，群体结构简单，遗传变异比较丰富，可以用于半野生棉目标性状的关联分析。应用关联分析，在3个年份环境下重复检测出12个与棉仁含油量显著相关的位点（P＜0.05），这12个位点可能与含油量性状存在稳定的关联，可为棉仁含油量性状的分子标记辅助选择提供依据，为开展半野生棉其它性状的关联分析提供参考。阔叶棉141平均棉仁含油量为41.90%，为高油份含量品种的遗传改良提供理想材料。

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