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植物学报  2015 

连续光照转暗培养联合药物处理实现衣藻细胞的高水平同步化

DOI: 10.3724/SP.J.1259.2015.00363, PP. 363-371

Keywords: 同步化培养,莱茵衣藻,流式细胞术,连续光照转暗培养,细胞周期阻断剂

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Abstract:

?单细胞衣藻(Chlamydomonas)是光合作用和植物细胞周期等生物学过程研究的一个重要模式系统,同步化培养是进行相关研究的必要手段。该研究探索了连续光照转暗培养联合细胞周期阻断剂实现莱茵衣藻(Chlamydomonasreinhardtii)细胞高水平同步化的新方法,并利用流式细胞术对同步化程度进行了精确的分析。结果表明,连续光照转暗培养或联合S期阻断剂可以使衣藻细胞同步化到G1期或G1/S期边界;连续光照转暗培养联合M期阻断剂或者在“加入-释放”S期阻断剂后再加入M期阻断剂可以使衣藻细胞同步化到M期,同步化水平可达80%。具体的同步化培养步骤要根据研究对象(特别是某些衣藻突变株系)的特性和研究目的确定。

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