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植物学报  2015 

pH敏感型荧光蛋白及其在植物细胞生物学中的应用

DOI: 10.3724/SP.J.1259.2015.00394, PP. 394-404

Keywords: 胞吞,胞吐,pH值活体测量,分子标记,pHluorin

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Abstract:

?pH敏感型荧光蛋白,即pHluorin,是荧光强度及光谱特征随环境pH值的变化而改变的一类荧光蛋白。人们通过对密码子使用偏好和特定剪切位点的修饰,已使pHluorin及其衍生物成功地在动物、植物和真菌细胞中正常表达,为测量细胞内微环境pH值的变化,并研究活细胞内依赖或导致pH变化的生理过程提供了有力工具。该文总结了目前已报道的pH敏感型荧光蛋白的种类及特性,并对其在细胞生物学,特别是植物细胞生物学中的应用进行了详细介绍。随着报告基因技术及检测方法的不断改进,pHluorin将在植物科学领域发挥更大的作用。

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