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植物学报  2015 

利用RNA-seq技术分析淹水胁迫下转BnERF拟南芥差异表达基因

DOI: 10.3724/SP.J.1259.2015.00321, PP. 321-330

Keywords: RNA-seq,乙烯响应因子,缺氧胁迫,氧化胁迫,耐淹

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Abstract:

?为探究淹水胁迫下BnERF调节的耐淹防御相关途径,应用RNA-seq技术,对淹水6小时后的拟南芥(Arabidopsisthaliana)野生型(WT)和转BnERF株系(E33)幼苗进行基因表达分析。结果表明,淹水3天后,E33表现出较强的耐淹性,地上部生长状况和根系发育均明显强于野生型。E33幼苗未淹水处理时相对于野生型单独上调的基因有9个,4个为膜结合蛋白,其中2个参与MAPK级联途径,其它5个参与氧化胁迫及水分调节途径;与未淹水野生型相比,无论是未淹水处理还是淹水6小时后的E33幼苗中缺氧响应、抗氧化防护及细胞、器官发育相关基因的表达量均上调。另外,淹水6小时后E33的差异基因并未完全覆盖淹水6小时后野生型的差异基因;E33幼苗中缺氧响应、氧化胁迫响应、能量的产生与转变、乙醇代谢途径中的基因以及乙烯响应因子基因的表达量都明显高于野生型。上述结果表明,BnERF直接或间接调节植物的淹水胁迫相关生理代谢途径,参与淹水胁迫的防御过程。

References

[1]  9 Fujimoto SY, Ohta M, Usui A, Shinshi H, Ohme-Takagi M (2000). Arabidopsis ethylene-responsive element binding factors act as transcriptional activators or repressors of GCC box-mediated gene expression. Plant Cell 12, 393-404.
[2]  10 Fukao T, Bailey-Serres J (2008). Submergence tolerance conferred by Sub1A is mediated by SLR1 and SLRL1 restriction of gibberellin responses in rice. Proc Natl Acad Sci USA 105, 16814-16819.
[3]  11 Fukao T, Xu KN, Ronald PC, Bailey-Serres J (2006). A variable cluster of ethylene response factor-like genes regulates metabolic and developmental acclimation responses to submergence in rice. Plant Cell 18, 2021- 2034.
[4]  12 Grichko VP, Glick BR (2001). Ethylene and flooding stress in plants. Plant Physiol Bioche 39, 1-9.
[5]  13 Jackson MB, Colmer TD (2005). Response and adaptation by plants to flooding stress. Ann Bot 96, 501-505.
[6]  14 Jaglo KR, Kleff S, Amundsen KL, Zhang X, Haake V, Zhang JZ, Deits T, Thomashow MF (2001). Components of the Arabidopsis C-repeat/dehydration-responsive element binding factor cold-response pathway are conserved in Brassica napus and other plant species. Plant Physiol 127, 910-917.
[7]  15 Jung KH, Seo YS, Walia H, Cao PJ, Fukao T, Canlas PE, Amonpant F, Bailey-Serres J, Ronald PC (2010). The submergence tolerance regulator Sub1A mediates stress- responsive expression of AP2 / ERF transcription factors. Plant Physiol 152, 1674-1692.
[8]  16 Kohorn BD, Johansen S, Shishido A, Todorova T, Martinez R, Defeo E, Obregon P (2009). Pectin activation of MAP kinase and gene expression is WAK2 dependent. Plant J 60, 974-982.
[9]  17 Licausi F, van Dongen JT, Giuntoli B, Novi G, Santaniello A, Geigenberger P, Perata P (2010). HRE1 and HRE2 , two hypoxia-inducible ethylene response factors, affect anaerobic responses in Arabidopsis thaliana . Plant J 62, 302-315.
[10]  18 Nakano T, Suzuki K, Fujimura T, Shinshi H (2006). Genome-wide analysis of the ERF gene family in Arabidopsis and rice. Plant Physiol 140, 411-432.
[11]  19 Ouaked F, Rozhon W, Lecourieux D, Hirt H (2003). A MAPK pathway mediates ethylene signaling in plants. EMBO J 22, 1282-1288.
[12]  20 Qi XH, Xu XW, Lin XJ, Zhang WJ, Chen XH (2012). Identification of differentially expressed genes in cucumber ( Cucumis sativus L.) root under waterlogging stress by digital gene expression profile. Genomics 99, 160-168.
[13]  21 Sakuma Y, Liu Q, Dubouzet JG, Abe H, Shinozaki K, Yamaguchi-Shinozaki K (2002). DNA-binding specificity of the ERF/AP2 domain of Arabidopsis DREBs, transcription factors involved in dehydration- and cold-inducible gene expression. Biochem Bioph Res Co 290, 998-1009.
[14]  22 Savitch LV, Allard G, Seki M, Robert LS, Tinker NA, Huner NPA, Shinozaki K, Singh J (2005). The effect of overexpression of two Brassica CBF / DREB1 -like trans- cription factors on photosynthetic capacity and freezing tolerance in Brassica napus . Plant Cell Physiol 46, 1525- 1539.
[15]  25 Xiong AS, Jiang HH, Zhuang J, Peng RH, Jin XF, Zhu B, Wang F, Zhang J, Yao QH (2013). Expression and function of a modified AP2/ERF transcription factor from Brassica napus enhances cold tolerance in transgenic Arabidopsis. Mol Biotechnol 53, 198-206.
[16]  26 Xu KN, Xu X, Fukao T, Canlas P, Maghirang-Rodriguez R, Heuer S, Ismail AM, Bailey-Serres J, Ronald PC, Mackill DJ (2006). Sub1A is an ethylene-response- factor-like gene that confers submergence tolerance to rice. Nature 442, 705-708.
[17]  27 Zhang GY, Chen M, Chen XP, Xu ZS, Guan S, Li LC, Li AL, Guo JM, Mao L, Ma YZ (2008). Phylogeny, gene structures, and expression patterns of the ERF gene family in soybean ( Glycine max L.). J Exp Bot 59, 4095-4107.
[18]  28 Zhao TJ, Sun S, Liu Y, Liu JM, Liu Q, Yan YB, Zhou HM (2006). Regulating the drought-responsive element (DRE)- mediated signaling pathway by synergic functions of trans -active and trans -inactive DRE binding factors in Brassica napus . J Biol Chem 281, 10752-10759.
[19]  1 肖文娟, 宾金华, 武波 (2004). 植物体中的MAPK. 植物学通报 21, 205-215.
[20]  2 张计育, 王庆菊, 郭忠仁 (2012). 植物AP2/ERF类转录因子研究进展. 遗传 34, 835-847.
[21]  3 Alam I, Lee DG, Kim KH, Park CH, Sharmin SA, Lee H, Oh KW, Yun BW, Lee BH (2010). Proteome analysis of soybean roots under waterlogging stress at an early vegetative stage. J Biosci 35, 49-62.
[22]  4 Ashburner M, Ball CA, Blake JA, Botstein D, Butler H, Cherry JM, Davis AP, Dolinski K, Selina S, Dwight SS, Eppig JT, Harris MA, Hill DP, Issel-Tarver L, Kasarskis A, Lewis S, Matese JC, Richardson JE, Ringwald M, Rubin GM, Sherlock G (2000). Gene ontology: tool for the unification of biology. Nat Genet 25, 25-29.
[23]  5 Cao YF, Song FM, Goodman RM, Zheng Z (2006). Molecular characterization of four rice genes encoding ethy- lene-responsive transcriptional factors and their expressions in response to biotic and abiotic stress. J Plant Physiol 163, 1167-1178.
[24]  6 Christianson JA, Liewellyn DJ, Dennis ES, Wilson IW (2010). Global gene expression responses to waterlogging in roots and leaves of cotton ( Gossypium hirsutum L.). Plant Cell Physiol 51, 21-37.
[25]  7 Clough SJ, Bent AF (1998). Floral dip: a simplified method for Agrobacterium -mediated transformation of Arabidopsis thaliana . Plant J 16, 735-743.
[26]  8 Dennis ES, Dolferus R, Ellis M, Rahman M, Wu Y, Hoeren FU, Grover A, Ismond KP, Good AG, Peacock WJ (2000). Molecular strategies for improving waterlogging tolerance in plants. J Exp Bot 51, 89-97.
[27]  23 Steinwand BJ, Kieber JJ (2010). The role of receptor-like kinases in regulating cell wall function. Plant Physiol 153, 479-484.
[28]  24 Wu LJ, Zhang ZJ, Zhang HW, Wang XC, Huang RF (2008). Transcriptional modulation of ethylene response factor protein JERF3 in the oxidative stress response enhances tolerance of tobacco seedlings to salt, drought, and freezing. Plant Physiol 148, 1953-1963.
[29]  29 Zhuang J, Chen JM, Yao QH, Xiong F, Sun CC, Zhou XR, Zhang J, Xiong AS (2011). Discovery and expression profile analysis of AP2/ERF family genes from Triticum aestivum . Mol Biol Rep 38, 745-753.
[30]  30 Zou XL, Tan XY, Hu CW, Zeng L, Lu GY, Fu GP, Cheng Y, Zhang XK (2013). The transcriptome of Brassica napus L. roots under waterlogging at the seedling stage. Int J Mol Sci 14, 2637-2651.

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