全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...
植物学报  2015 

3D-SIM结构照明超分辨率显微镜实现蛋白质在植物亚细胞器内的定位

DOI: 10.11983/CBB15038, PP. 495-503

Keywords: 荧光蛋白,OMX,植物亚细胞定位,超分辨率显微镜

Full-Text   Cite this paper   Add to My Lib

Abstract:

?基因表达产物蛋白质的亚细胞定位是解析基因生物学功能的重要证据之一。近年来出现的超分辨率光学成像技术已成功应用于人类和动物细胞中,预示着显微成像技术继激光共聚焦技术后的又一重要进步。由于植物细胞的特殊性和成像技术的研发取向,超分辨率光学成像技术在植物细胞蛋白质亚细胞定位的应用尚未见报道。该研究利用DeltaVisionOMX显微镜技术,克服了叶绿体基粒中叶绿素自发荧光与融合蛋白荧光不易区分的缺陷,解决了受分辨率局限无法将植物细胞中蛋白质在亚细胞器内可视化精确定位的技术难题,成功地将植物蔗糖合成酶ZmSUS-SH1定位在烟草表皮细胞叶绿体基粒周围。该研究同时建立了一套基于撕片制片法的简便OMX显微镜制片方法,并针对OMX显微成像技术在植物细胞中蛋白质亚细胞定位的应用进行了讨论。

References

[1]  1 龚燕华, 彭小忠 (2010). 蛋白质相互作用及亚细胞定位原理与技术. 北京: 中国协和医科大学出版社. pp. 210-234.
[2]  2 李楠, 王黎明, 杨军 (1996). 激光共聚焦显微镜的原理和应用. 军医进修学院学报 17, 232-234.
[3]  3 邢浩然, 刘丽娟, 刘国振 (2006). 植物蛋白质的亚细胞定位研究进展. 华北农学报 21(增刊), 1-6.
[4]  4 赵启韬, 苗俊英 (2003). 激光共聚焦显微镜在生物医学研究中的应用. 北京生物医学工程 22, 52-54.
[5]  5 Brown ACN, Oddos S, Dobbie IM, Alakoskela JM, Parton RM, Eissmann P, Neil MAA, Dunsby C, French PMW, Davis I, Davis DM (2011). Remodelling of cortical actin where lytic granules dock at natural killer cell immune synapses revealed by super resolution microscopy. PLoS Biol 9, 1-18.
[6]  6 Coltharp C, Xiao J (2012). Superresolution microscopy for microbiology. Cell Microbiol 14, 1808-1818.
[7]  7 Dickerson D (2010). A study of chromatin dynamics during transcription by fluorescence light microscopy. Doctoral Thesis. Dundee: University of Dundee.
[8]  8 Dobbie IM, King E, Parton RM, Carlton PM, Sedat JW, Swedlow JR, Davis I (2011). OMX: a new platform for multimodal, multichannel wide-field imaging. In: Goldmam RD, ed. Live Cell Imaging, 2nd edn. Cold Spring Harbor: Cold Spring Harbor Laboratory Press. pp. 898-909.
[9]  14 Seabold GK, Wang PY, Petralia RS, Chang K, Zhou A, McDermott MI, Wang YX, Milgram SL, Wenthold RJ (2012). Dileucine and PDZ-binding motifs mediate synaptic adhesion-like molecule 1 (SALM1) trafficking in hippocampal neurons. J Biol Chem 287, 4470-4484.
[10]  15 Swedlow JR (2012). Innovation in biological microscopy: current status and future directions. BioEssays 34, 333-340.
[11]  16 Tanos BE, Yang HJ, Soni R, Wang WJ, Macaluso FP, Asara JM, Tsou MFB (2013). Centriole distal appendages promote membrane docking, leading to cilia initiation. Genes Dev 27, 163-168.
[12]  17 van de Corput MPC, de Boer E, Knoch TA, van Cappellen WA, Quintanilla A, Ferrand L, Grosveld FG (2012). Super-resolution imaging reveals three-dimensional folding dynamics of the β-globin locus upon gene activation. J Cell Sci 125, 4630-4639.
[13]  9 Gustafsson MGL (2008). Super-resolution light microscopy goes live. Nat Methods 5, 385-387.
[14]  10 Gustafsson MGL, Shao L, Carlton PM, Wang CJR, Golubovskaya IN, Cande WZ, Agard DA, Sedat JW (2008). Three-dimensional resolution doubling in wide- field fluorescence microscopy by structured illumination. Biophys J 94, 4957-4970.
[15]  11 Masters BR (2010). The development of fluorescence microscopy. eLS 1-9, doi:10.1002/9780470015902. a0022- 093.
[16]  12 Minden JS, Agard DA, Sedat JW, Alberts BM (1989). Direct cell lineage analysis in Drosophila melanogaster by time-lapse, three-dimensional optical microscopy of living embryos. J Cell Biol 109, 505-516.
[17]  13 Schermelleh L, Carlton PM, Haase S, Shao L, Winoto L, Kner P, Burke B, Cardoso MC, Agard DA, Gustafsson MGL, Leonhardt H, Sedat JW (2008). Subdiffraction multicolor imaging of the nuclear periphery with 3D structured illumination microscopy. Science 320, 1332- 1336.
[18]  18 Weil TT, Xanthakis D, Parton R, Dobbie I, Rabouille C, Gavis ER, Davis I (2010). Distinguishing direct from indirect roles for bicoid mRNA localization factors. Deve- lopment 137, 169-176.
[19]  19 Zheng CY, Wang YX, Kachar B, Petralia RS (2011). Differential localization of SAP102 and PSD-95 is revealed in hippocampal spines using super-resolution light microscopy. Commun Integr Biol 4, 104-105.

Full-Text

Contact Us

[email protected]

QQ:3279437679

WhatsApp +8615387084133