全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...

甜菜夜蛾对氯虫苯甲酰胺抗性种群选育及鱼尼丁受体基因表达特征

, PP. 425-431

Keywords: 甜菜夜蛾,氯虫苯甲酰胺,抗性选育,鱼尼丁受体基因

Full-Text   Cite this paper   Add to My Lib

Abstract:

为明确甜菜夜蛾Spodopteraexigua对氯虫苯甲酰胺的抗性发展及抗性种群中鱼尼丁受体(ryanodinereceptor,RyR)基因的表达量变化,室内采用饲料混毒法进行甜菜夜蛾对氯虫苯甲酰胺的抗性选育,荧光定量PCR技术研究了抗性和敏感种群之间RyR基因mRNA表达量的差异.结果表明,室内选育31代后获得一个抗性倍数为105.60倍的甜菜夜蛾抗性种群,其mRNA表达量在甜菜夜蛾不同发育阶段及抗性种群和敏感种群之间均存在差异,以表达量最低的卵期作为对照,抗性种群中1龄幼虫表达量最高,是卵期的154.58倍;其次是雄性成虫,其表达量是卵期的101.51倍;2~5龄幼虫分别是卵期的59.56、35.35、72.99和19.84倍.抗性种群中1、2和4龄幼虫mRNA表达量分别是敏感种群的5.99、2.79和2.14倍,其余阶段低于敏感种群.表明甜菜夜蛾对氯虫苯甲酰胺的抗药性可能主要表现在幼虫阶段,RyR基因的表达量变化与氯虫苯甲酰胺诱导有关.

References

[1]  Che WN, Shi T, Wu YD, Yang YH. 2013. Insecticide resistance status of field populations of Spodoptera exigua (Lepidoptera: Noctuidae) from China. Journal of Economic Entomology, 106(4): 1855-1862
[2]  Chen Q, Huang SJ, Qin WJ. 2011. Sublethal effects of chlorantraniliprole on Spodoptera exigua. Acta Agriculturae Universitatis Jiangxiensis (Natural Sciences Edition), 33(4): 690-695 (in Chinese) [陈琼, 黄水金, 秦文婧. 2011. 氯虫苯甲酰胺对甜菜夜蛾的亚致死效应研究. 江西农业大学学报, 33(4): 690-
[3]  Cui L, Yang DB, Yan XJ, Rui CH, Wang ZY, Yuan HZ. 2013. Molecular cloning, characterization and expression profiling of a ryanodine receptor gene in Asian corn borer, Ostrinia furnacalis (Guenée). PLoS ONE, 8(10): e75825
[4]  Du JH, Yu WL, Wang M, Zhang CG, Mu W. 2013. Selective toxicity of three amide pesticides to black cutworm Agrotis ypsilon and earthworm Eisenia foetida. Journal of Plant Protection, 40(3): 266-272 (in Chinese) [杜军辉, 于伟丽, 王猛, 张灿光, 慕卫. 2013. 三种双酰胺类杀虫剂对小地老虎和蚯蚓的选择毒性. 植物保护学报, 2013, 40(3): 266-
[5]  Gong W, Yan HH, Gao L, Guo YY, Xue CB. 2014. Chlorantraniliprole resistance in the diamondback moth (Lepidoptera: Plutellidae). Journal of Economic Entomology, 107(2): 806-814
[6]  Greenberg SM, Sappington TW, Legaspi BC, Liu TX, Sétamou M. 2001. Feeding and life history of Spodoptera exigua (Lepidoptera: Noctuidae) on different host plants. Annals of the Entomological Society of America, 94(4): 566-575
[7]  Guo L, Tang BZ, Dong W, Liang P, Gao XW. 2012. Cloning, characterisation and expression profiling of the cDNA encoding the ryanodine receptor in diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae). Pest Management Science, 68(12): 1605-1614
[8]  Guo L, Wang Y, Zhou XG, Li ZY, Liu SZ, Liang P, Gao XW. 2014. Functional analysis of a point mutation in the ryanodine receptor of Plutella xylostella (L.) associated with resistance to chlorantraniliprole. Pest Management Science, 70(7): 1083 -1089
[9]  Hasan G, Rosbash M. 1992. Drosophila homologs of two mammalian intracellular Ca(2+)-release channels: identification and expression patterns of the inositol 1,4,5-triphosphate and the ryanodine receptor genes. Development, 116(4): 967-975
[10]  He YP, Zhang JF, Chen JM. 2014. Effect of synergists on susceptibility to chlorantraniliprole in field populations of Chilo suppressalis (Lepidoptera: Pyralidae). Journal of Economic Entomology, 107(2): 791-796
[11]  Lahm GP, Cordova D, Barry JD. 2009. New and selective ryanodine receptor activators for insect control. Bioorganic & Medicinal Chemistry, 17(12): 4127-4133
[12]  Lai TC, Li J, Su JY. 2011. Monitoring of beet armyworm Spodoptera exigua (Lepidoptera: Noctuidae) resistance to chlorantraniliprole in China. Pesticide Biochemistry and Physiology, 101(3): 198-205
[13]  Lai TC, Su JY. 2011. Assessment of resistance risk in Spodoptera exigua (Hübner) (Lepidoptera: Noctuidae) to chlorantraniliprole. Pest Management Science, 67(11): 1468-1472
[14]  Li XC, Degain BA, Harpold VS, Mar?on PG, Nichols RL, Fournier AJ, Naranjo SE, Palumbo JC, Ellsworth PC. 2012. Baseline susceptibilities of B- and Q-biotype Bemisia tabaci to anthranilic diamides in Arizona. Pest Management Science, 68(1): 83-91
[15]  Li XH, Gao M, Zhou C, Mu W. 2012. The susceptible strain of Spodoptera exigua and the susceptivity baseline to 12 insecticides. Journal of Plant Protection, 39(4): 376-380 (in Chinese) [李秀环, 高明, 周超, 慕卫. 2012. 甜菜夜蛾敏感品系的获得及其对12种杀虫剂的敏感基线. 植物保护学报, 39(4): 376-
[16]  Moulton JK, Pepper DA, Jansson RK, Dennehy TJ. 2002. Pro-active management of beet-armyworm (Lepidoptera: Noctuidae) resistance to tebufenozide and methoxyfenozide baseline monitoring, risk assessment, and isolation of resistance. Journal of Economic Entomology, 95(2): 414-424
[17]  Osoria A, Martinez AM, Schneider MI, Díaz O, Corrales JL, Avilés MC, Smagghe G, Pineda S. 2008. Monitoring of beet armyworm resistance to spinosad and methoxyfenozide in Mexico. Pest Management Science, 64(10): 1001-1007
[18]  Sun LN, Cui L, Rui CH, Yan XJ, Yang DB, Yuan HZ. 2012. Modulation of the expression of ryanodine receptor mRNA from Plutella xylostella as a result of diamide insecticide application. Gene, 511(2): 265-273
[19]  Sun LN, Yang DB, Rui CH, Cui L, Yuan HZ. 2012. Effects of chlorantraniliprole on expression abundance of ryanodine receptor gene mRNA from Plutella xylostella. Chinese Journal of Pesticide Science, 14(2): 136-142 (in Chinese) [孙丽娜, 杨代斌, 瑞昌辉, 崔丽, 袁会珠. 2012. 氯虫苯甲酰胺对小菜蛾鱼尼丁受体基因mRNA表达量的影响. 农药学学报, 14(2): 136-
[20]  Tohnishi M, Nakao H, Furuya T, Seo A, Kodama H, Tsubata K, Fujioka S, Kodama H, Hirooka T, Nishimatsu T. 2005. Flubendiamide, a novel insecticide with high activity against Lepidoptera insect pests. Journal of Pesticide Science, 30(4): 354-360
[21]  更多...
[22]  Troczka B, Zimmer CT, Elias J, Schorn C, Bass C, Davies TG, Field LM, Williamson MS, Slater R, Nauen R. 2012. Resistance to diamide insecticides in diamondback moth, Plutella xylostella (Lepidoptera: Plutellidae) is associated with a mutation in the membrane-spanning domain of the ryanodine receptor. Insect Biochemistry and Molecular Biology, 42(11): 873-880
[23]  Wan PJ, Guo WY, Yang Y, Lü FG, Lu WP, Li GQ. 2014. RNAi suppression of the ryanodine receptor gene results in decreased susceptibility to chlorantraniliprole in Colorado potato beetle Leptinotarsa decemlineata. Journal of Insect Physiology, 63: 48 -55
[24]  Wang KY, Jiang XY, Yi MQ, Chen BK, Xia XM. 2002. Insecticide resistance and its mechanism of Spodoptera exigua. Journal of Plant Protection, 29(3): 229-234 (in Chinese) [王开运, 姜兴印, 仪美芹, 陈丙坤, 夏晓明. 2002. 甜菜夜蛾抗药性及其机理. 植物保护学报, 29(3): 229-
[25]  Wang XL, Wu YD. 2012. High levels of resistance to chlorantraniliprole evolved in field populations of Plutella xylostella. Journal of Economic Entomology, 105(3): 1019-1023
[26]  Xi DQ. 2010. Study on the control effect of chlorantraniliprole against Laphygma exigua Hübner. Northern Horticulture, (12): 158-159 (in Chinese) [席敦芹. 2010. 氯虫苯甲酰胺防治甜菜夜蛾药效试验研究. 北方园艺, (12): 158-
[27]  Xing J, Liang P, Gao XW. 2011. Effects of sublethal concentrations of chlorantraniliprole on insecticide susceptibility and detoxifying enzyme activity in Plutella xylostella. Chinese Journal of Pesticide Science, 13(5): 464-470 (in Chinese) [邢静, 梁沛, 高希武. 2011. 亚致死浓度氯虫苯甲酰胺对小菜蛾药剂敏感度和解毒酶活性的影响. 农药学学报, 13(5): 464-
[28]  Yang Y, Wan PJ, Hu XX, Li GQ. 2014. RNAi mediated knockdown of the ryanodine receptor gene decreases chlorantraniliprole susceptibility in Sogatella furcifera. Pesticide Biochemistry and Physiology, 108: 58-65
[29]  Zhang Q, Wang SL, Yang YY, Dong JF. 2010. Effects of chlorantraniliprole on baseline sensitivity and weight of larval of Spodoptera exigua. Journal of Changjiang Vegetables, (18): 87-89 (in Chinese) [张琴, 王少丽, 杨愿愿, 董钧锋. 2010. 氯虫苯甲酰胺对甜菜夜蛾敏感基线的测定和幼虫体重的影响. 长江蔬菜, (18): 87-
[30]  Zhang RM, Dong JF, Chen JH. 2013. The sublethal effects of chlorantraniliprole on Helicoverpa armigera (Lepidoptera: Noctuidae). Journal of Integrative Agriculture, 12(3): 457-466
[31]  Zhou C, Wang HN, Li XH, Wang W, Mu W. 2011. Comparison of the toxicity of chlorantraniliprole and flubendiamide to different developmental stages of Spodoptera exigua. Journal of Plant Protection, 38(4): 344-350 (in Chinese) [周超, 王海娜, 李秀环, 王伟, 慕卫. 2011. 氯虫苯甲酰胺和氟虫双酰胺对不同虫态甜菜夜蛾的毒力作用. 植物保护学报, 38(4): 344 -

Full-Text

Contact Us

[email protected]

QQ:3279437679

WhatsApp +8615387084133