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土壤环境对大豆疫霉Phytophthorasojae卵孢子萌动的影响

, PP. 370-375

Keywords: 大豆疫霉,增强型绿色荧光蛋白,土壤环境,卵孢子萌动

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Abstract:

为明确土壤环境对大豆疫霉Phytophthorasojae卵孢子萌动的影响,将增强型绿色荧光蛋白标记的大豆疫霉卵孢子以2500个卵孢子/g干土的比例接种于无菌黑土中,荧光显微镜下计数卵孢子的萌动率以明确其最适宜的土壤温度和含水量;在此基础上,从5种类型土壤和5种轮作体系土壤中筛选适宜卵孢子萌动的土壤环境.结果表明,25℃土壤温度和30%土壤含水量最适合大豆疫霉卵孢子萌动,萌动率为95.78%;黑土和盐碱土分别是最适合和最不适合卵孢子萌动的土壤类型,萌动率分别为94.94%和14.67%;卵孢子萌动率与土壤有机质含量、pH值和Ca2+含量间无明显相关性.大豆连作田和玉米连作田土壤适合卵孢子萌动,萌动率为96.33%和95.00%,小麦连作田土壤不适合卵孢子萌动,萌动率仅为39.33%.

References

[1]  Zuo YH, Zang ZJ, Han WG, Liu TR. 2002b. Studies on germinating condition of oospores of Phytophthora sojae. Soybean Science, 21(2): 101-105 (in Chinese) [左豫虎, 藏忠婧, 韩文革, 刘惕若. 2002b. 影响大豆疫霉菌(Phytophthora sojae)卵孢子萌发的条件. 大豆科学, 21(2): 101-
[2]  Zuo YH, Xue CS, Liu TR. 2002a. Influence of environmental conditions upon development of Phytophthora root rot of soybean seedling. Acta Agriculturae Boreali-Sinica, 17(4): 93-95 (in Chinese) [左豫虎, 薛春生, 刘惕若. 2002a. 环境条件对大豆幼苗疫病发生的影响. 华北农学报, 17(4): 93-
[3]  Chen QM, Xiao CX, Sun QQ, Wen JZ. 2015. Overwintering survival rate of Phytophthora sojae oospores in soil in Heilongjiang Province. Journal of Plant Protection, 42(1): 72-78 (in Chinese)[陈秋明, 肖彩霞, 孙欠欠, 文景芝. 2015. 大豆疫霉Phytophthora sojae卵孢子在黑龙江省土壤中的越冬存活率. 植物保护学报, 42(1): 72-
[4]  Chu L, He B. 2009. Determining method of soil cation exchange capacity. Heilongjiang Environmental Journal, 33(1): 81-83 (in Chinese) [褚龙, 贺斌. 2009. 土壤阳离子交换量的测定方法. 黑龙江环境通报, 33(1): 81-
[5]  Duniway JM. 1979. Water relations of water molds. Annual Review of Phytopathology, 17: 431-460
[6]  Huo XT, Wang WL. 1998. Improvement on determine method to soil organic matter content. Acta Agriculturae Universitatis Henanensis, 32(1): 86-89 (in Chinese) [霍晓婷, 王文亮. 1998. 土壤有机质含量测定方法改进的研究. 河南农业大学学报, 32(1): 86-
[7]  Kuhajek JM, Jeffers SN, Slattery M, Wedge DE. 2003. A rapid microbioassay for discovery of novel fungicides for Phytophthora spp. Phytopathology, 93(1): 46-53
[8]  Li Q, Wen HC, Hu CY. 2007. Difference between international and domestic methods in determining soil pH. Soils, 39(3): 488-491 (in Chinese) [李强, 文唤成, 胡彩荣. 2007. 土壤pH值的测定国际国内方法差异研究. 土壤, 39(3): 488-
[9]  Schmittenner AF. 1985. Problems and progress in control of Phytophthora root rot of soybean. Plant Disease, 69(4): 362-368
[10]  Schmitthenner AF, Bhat RG. 1994. Useful methods for studying Phytophthora in the laboratory. Wooster: Ohio Agricultural Research Development Center, Department of Plant Pathology, Special Circular 143, pp. 1-10
[11]  Shen CY, Su YC. 1991. Discovery and preliminary studies of Phytophthora megasperma on soybean in China. Acta Phytopathologica Sinica, 21(4): 298 (in Chinese) [沈崇尧, 苏彦纯. 1991. 中国大豆疫霉病菌的发现及初步研究. 植物病理学报, 21(4):
[12]  Su YC, Shen CY. 1993. The discovery and biological characteristics studies of Phytophthora megasperma f. sp. glycinea on soybean in China. Acta Phytopathologica Sinica, 23(4): 341-347 (in Chinese) [苏彦纯, 沈崇尧. 1993. 大豆疫霉病菌在中国的发现及其生物学特性的研究. 植物病理学报, 23(4): 341-
[13]  Wang F, Song CY, Han XZ. 2006. Characteristics of soil temperature dynamics in the black soil area of Northeast China. Heilongjiang Agricultural Sciences, (6): 31-33 (in Chinese) [王风, 宋春雨, 韩晓增. 2006. 东北黑土区土壤温度变化特征. 黑龙江农业科学, (6): 31-
[14]  Wen JZ. 2003. Detection and identification of Phytophthora sojae and transmission of Phytophthora root rot of soybean. Acta Phytopathologica Sinica, 33(2): 191 (in Chinese) [文景芝. 2003. 大豆疫霉根腐病菌检测鉴定方法及病害传播途径研究. 植物病理学报, 33(2):
[15]  Wen JZ, Li G, Zhang QF, Wu FZ. 2007b. Effect of fertilization on cucumber rhizospheric microbial communities in greenhouse. China Vegetables, (12): 11-14 (in Chinese) [文景芝, 李刚, 张齐凤, 吴凤芝. 2007b. 施肥对大棚黄瓜根际微生物群落结构和数量消长的影响. 中国蔬菜, (12): 11-
[16]  Wen JZ, Yang MX, Hao ZN, Liu CL. 2007a. Factors affecting viability and germination of oospores of Phytophthora sojae. Chinese Journal of Oil Crop Sciences, 29(3): 322-327 (in Chinese) [文景芝, 杨明秀, 郝中娜, 刘春来. 2007a. 影响大豆疫霉菌Phytophthora sojae卵孢子生活力和萌发的因素. 中国油料作物学报, 29(3): 322-
[17]  Wrather JA, Anderson TR, Arsyad DM, Tan Y, Ploper LD, Porta-Puglia A, Ram HH, Yorinori JT. 2001. Soybean disease loss estimates for the top ten soybean-producing countries in 1998. Canadian Journal of Plant Pathology, 23(2): 115-121
[18]  Wrather JA, Koenning SK. 2006. Estimates of disease effects on soybean yields in the United States 2003 to 2005. Journal of Nematology, 38(2): 173-180
[19]  Wu H, Wang LH, Wu XH, Zheng XB. 2002. A preliminary study on the fitness of Phytophthora sojae to the environment in Jiangsu Province, China. Journal of Nanjing Agricultural University, 25(1): 39-42 (in Chinese) [吴浩, 王良华, 吴新华, 郑小波. 2002. 大豆疫霉在江苏省适生性的初步研究. 南京农业大学学报, 25(1): 39-
[20]  Xing LJ, Li MC. 1999. General plant pathology. Beijing: Higher Education Press, pp. 70 (in Chinese) [邢来君,李明春. 1999. 普通真菌学. 北京: 高等教育出版社, pp.
[21]  Yu X, Yang DL, Wang JF, Chen JH. 2009. Progress of research on Phytohthora root rot of soybean. Plant Quarantine, 23(5): 47-50 (in Chinese) [余霞, 杨丹玲, 王俊峰, 陈进会. 2009. 大豆疫霉病研究进展. 植物检疫, 23(5): 47-
[22]  Zhang JH, Liu XM, Zhang YJ, Zhang HJ. 2008. Effect of inoculum density, soil water content and soil temperature on soybean Phytophthora sojae. Chinese Journal of Oil Crop Sciences, 30(3): 342-345 (in Chinese) [张俊华, 刘学敏, 张艳菊, 张海军. 2008. 接种体密度、土壤含水量和土壤温度对大豆疫霉根腐病发生的影响. 中国油料作物学报, 30(3): 342-
[23]  更多...
[24]  Zhu ZD, Wang XM, Dai FC. 1999. The occurrence of soybean Phytophthora root rot in China and its countermeasures. Plant Protection, 25(3): 47-49 (in Chinese) [朱振东, 王晓鸣, 戴发超. 1999. 大豆疫霉根腐病在我国的发生及防治对策. 植物保护, 25(3): 47-

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