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克雷伯氏肺炎杆菌内抗亚碲酸盐蛋白质TerZ延伸环降低对钙离子亲合性Hot!

DOI: 10.11938/cjmr20150213, PP. 308-317

Keywords: 钙离子结合蛋白质,抗亚碲酸盐,钙离子传感器,化学位移指数,同源模拟

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Abstract:

亚碲酸盐是碲的一含氧阴离子,其对微生物具高度毒性.在许多的致病菌内已经鉴定出数个抗亚碲酸盐基因(terZABCDEF).之前,作者解出抗亚碲酸盐蛋白质TerD液体核磁共振结构并指出在细菌内TerD可能是一钙离子传感器.TerZ与TerD在序列上有40%相同性,其包括了一额外的9氨基酸片段L36-N44,并且显示出非常弱的钙离子亲合性.有趣的是,少了额外片段的TerZdel拥有与TerD可比较的钙离子亲合性.根据化学位移指数及同源模拟结果,此额外片段为一无二级结构且延伸的loop,可能扰乱钙离子结合位置的构形,同时也阻碍了钙离子接近其结合位置,因此大大降低钙离子亲合性.

References

[1]  Johnson B A, Blevins R A. NMR View: A computer program for the visualization and analysis of NMR data[J]. J Biomol NMR, 1994, 4(5): 603-614.
[2]  Biasini M, Bienert S, Waterhouse A, et al. SWISS-MODEL: modelling protein tertiary and quaternary structure using evolutionary information[J]. Nucleic Acids Res, 2014, 42(W1): W252-W258.
[3]  Laskowski R A, Macarthur M W, Moss D S, et al. Procheck: a program to check the stereochemical quality of protein structures[J]. J Appl Cryst, 1993, 26(0), 283-291.
[4]  Shen Y, Bax A. Protein backbone and sidechain torsion angles predicted from NMR chemical shifts using artificial neural networks[J]. J Biomol NMR, 2013, 56(3): 227-241.
[5]  Taylor D E. Bacterial tellurite resistance[J]. Trends Microbiol, 1999, 7(3): 111-115.
[6]  Chasteen T G, Fuentes D E, Tantalean J C, et al. Tellurite: history, oxidative stress, and molecular mechanisms of resistance[J]. FEMS Microbiol Rev, 2009, 33(4): 820-832.
[7]  Walter E G, Taylor D E. Plasmid-mediated resistance to tellurite: expressed and cryptic[J]. Plasmid, 1992, 27(1): 52-64.
[8]  Chen Y T, Chang H Y, Lai Y C, et al. Sequencing and analysis of the large virulence plasmid pLVPK of Klebsiella pneumoniae CG43[J]. Gene, 2004, 337: 189-198.
[9]  Whelan K F, Colleran E, Taylor D E. Phage inhibition, colicin resistance, and tellurite resistance are encoded by a single cluster of genes on the IncHI2 plasmid R478[J]. J Bacteriol, 1995, 177(17): 5 016-5 027.
[10]  Valkovicova L, Vavrova S M, Mravec J, et al. Protein-protein association and cellular localization of four essential gene products encoded by tellurite resistance-conferring cluster “ter” from pathogenic Escherichia coli[J]. Antonie Van Leeuwenhoek, 2013, 104(6): 899-911.
[11]  Anantharaman V, Iyer L M, Aravind L. Ter-dependent stress response systems: novel pathways related to metal sensing, production of a nucleoside-like metabolite, and DNA-processing[J]. Mol Biosyst, 2012, 8(12): 3 142-3 165.
[12]  Pan Y R, Lou Y C, Seven A B, et al. NMR structure and calcium-binding properties of the tellurite resistance protein TerD from Klebsiella pneumoniae[J]. J Mol Biol, 2011, 405(5): 1 188-1 201.
[13]  Pan Y R. Structure as a Guide to Function: NMR Studies on Tellurite Resistance Proteins from Klebsiella pneumoniae[D]. Hsinchu: Institute of Bioinformatics and Structural Biology, College of Life Science, “National Tsing Hua University”, 2011.
[14]  Wu K M, Li L H, Yan J J, et al. Genome sequencing and comparative analysis of Klebsiella pneumoniae NTUH-K2044, a strain causing liver abscess and meningitis[J]. J Bacteriol, 2009, 191(14): 4 492-4 501.
[15]  Schuck P. Size-distribution analysis of macromolecules by sedimentation velocity ultracentrifugation and lamm equation modeling[J]. Biophys J, 2000, 78(3): 1 606-1 619.
[16]  Delaglio F, Grzesiek S, Vuister G W, et al. NMRPipe: a multidimensional spectral processing system based on UNIX pipes[J]. J Biomol NMR, 1995, 6(3): 277-293.
[17]  Sali A, Blundell T L. Comparative protein modelling by satisfaction of spatial restraints[J]. J Mol Biol, 1993, 234(3): 779-815.
[18]  Morris A L, MacArthur M W, Hutchinson E G., et al. Stereochemical quality of protein structure coordinates[J]. Proteins, 1992, 12(4): 345-364.

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