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流式细胞仪检测高等植物细胞核DNA含量的方法

DOI: 10.11913/PSJ.2095-0837.2015.10126, PP. 126-131

Keywords: 流式细胞术,高等植物,细胞核DNA含量,DNA解离液

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Abstract:

?相对于动物和微生物而言,流式细胞术在植物科学上的应用会因植物组织与细胞(如细胞壁、中央液泡、特殊细胞器等)的特殊结构以及次生代谢产物等特殊成分,造成样品在前期处理、染色及测试等方面的困难,甚至导致检测失败或结果不准确。笔者在长期运用流式细胞仪测试工作中,积累了大量的植物样本检测经验,并参考国内外相关文献,总结出从植物取材、样品制备到植物细胞核DNA流式检测的方法和技巧,可为植物科学研究者及从事流式细胞检测的技术人员提供实验参考。

References

[1]  焦旭雯,赵树进.流式细胞术在高等植物研究中的应用[J].热带亚热带植物学报,2006,14(4): 354-358.
[2]  Galbraith DW, Lambert GM, Macas J, Dolezel J. Analysis of nuclear DNA content and ploidy in higher plants[J]. Curr Protoc Cytom, 2001,7(6): 1-22.
[3]  Dolezel J, Bartos J. Plant DNA flow cytometry and estimation of nuclear genome size[J]. Ann Bot, 2005, 95(1): 99-110.
[4]  Hare EE, Johnston JS. Genome size determination using flow cytometry of propidium iodide-stained nuclei[J]. Methods Mol Biol, 2011,772: 3-12.
[5]  Satio H,Mizunashi K,Tanaka S, Adachi Y, Nakano M. Ploidy estimation in Hemerocallis species and cultivars by flow cytometry[J]. Scientia Horticulturae, 2003,97: 185-192.
[6]  Suda J, Trávnicek P. Reliable DNA Ploidy determination in dehydrated tissues of vascular plants by DAPI flow cytometry——new prospectsfor plant research[J]. Cytometry A, 2006,69(4): 273-280.
[7]  Loureiro J, Rodriguez E, Dolezel J, Santos C. Comparison of four nuclear isolation buffers for plant DNA flow cytometry[J]. Ann Bot, 2006, 98(3): 679-689.
[8]  Li ZZ, Man YP, Lan XY, Wang YC. Ploidy and phenotype variation of a natural Actnidia arguta population in the east of Daba Mountain located in a region of Shaanxi[J]. Scientia Horticulturae, 2013,161: 259-265.
[9]  Chen JJ, Liu XL, Zhu LY, Wang Y. Nuclear genome size estimation and karyotype analysis of Lycium species[J]. Scientia Horticulturae, 2013,151: 46-50.
[10]  Noirot M, Barre P, Duperray C, Louarn J, Hamon S. Effects of caffeine and chlorogenic acid on propidium iodide ac-cessibility to DNA:consequences on genome size evaluation in coffee tree[J]. Ann Bot, 2003, 92(2): 259-264.
[11]  Georgiev V, Weber J, Bley T, Pavlov A. Improved procedure for nucleus extraction for DNA measurements by flowcytometry of red beet (Beta vulgaris L.) hairy roots[J]. J Biosci Bioeng, 2009, 107(4): 439-441.
[12]  Roux N, Toloza A, Radecke Z, Zapata-Arias FJ, Dolezel J. Rapid detection of aneuploidy in musa using flow cytometry[J]. Plant Cell Rep, 2003,21(5): 483-490.
[13]  Phivnil K, Beppu K, Takamura T, Fukuda T, Kataoka I. Flow cytometric assessment of ploidy in native resources of Actinidia in Japan[J]. J Am Pom Soc, 2005, 59(1): 44-49.
[14]  Aliyu OM. Development of the flow cytometric protocol for ploidy analysis and determination of relative nuclear DNA content in cashew[J]. Am J Biochem Mol Biol, 2012,2(4): 200-215.
[15]  弓娜,田新民,周香艳,刘建全.流式细胞术在植物学研究中的应用——检测植物核DNA含量和倍性水平[J].中国农学通报,2011,27(9): 21-27.
[16]  Lee HC, Lin TY. Isolation of plant nuclei suitable for flow cytometry from recalcitrant tissue by use of a filtration column[J]. Plant Mol Bio Rep, 2005,23: 53-58.
[17]  于红梅,王静,赵密珍,孟宪凤.利用流式细胞仪检测草莓倍性方法的优化[J].南方农业学报,2012,43(10):1530-1533.
[18]  Cousin A, Heel K, Cowling W A, Nelson MN. An efficient high-throughput flow cytometric method for estimating DNA ploidy level in plants[J]. Cytometry, 2009,75(12): 1015-1019.
[19]  Dolezel J,Greilhuber J,Lucretti S,Meister A, Lysak MA, Nard L, Obermayer R. Plant genome size estimation by flow cytometry: inter-laboratory comparison[J]. Ann Bot, 1998,82(Supplement A): 17-26.

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