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水杨酸诱发的NO介导了丹参悬浮培养细胞中丹酚酸B的生物合成

DOI: 10.11913/PSJ.2095-0837.2015.10081, PP. 81-89

Keywords: 一氧化氮(NO),苯丙氨酸解氨酶,水杨酸,丹参,丹酚酸B,信号转导

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Abstract:

?水杨酸(SA)可诱导丹参悬浮培养细胞中一氧化氮(NO)产生、苯丙氨酸解氨酶(PAL)活化及丹酚酸B(SalB)的生物合成。为了阐明NO对丹参悬浮培养细胞中SalB生物合成的影响及作用机理,本实验利用NO供体硝普钠(SNP)、NO合成酶抑制剂L-NNA(Nω-nitro-L-arginine)、NO淬灭剂cPITO(carboxy-2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide)以及PAL抑制剂L-AOPP(L-2-aminooxygen-3-phenylacrylicacid)分别处理丹参悬浮培养细胞,并对其胞内NO水平、PAL活性和SalB积累量进行了检测。结果表明,硝普钠(SNP)处理显著促进了NO产生、PAL活性和SalB的积累,而L-NNA和cPITO抑制上述过程,说明NO诱发PAL活性提高并参与了SA诱导的SalB生物合成;L-AOPP显著抑制了PAL活性及SalB积累,却对NO产生没有显著影响,揭示NO位于PAL的上游。这说明SA诱发的NO产生、PAL活化及SalB合成之间存在因果关系,即NO通过激活PAL触发SalB生物合成。

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