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在基因家族背景下对四种植物中DGAT2的鉴定和序列分析

DOI: 10.11913/PSJ.2095-0837.2015.20186, PP. 186-194

Keywords: DGAT2,三酰甘油,限速酶,基因家族

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Abstract:

?二酰甘油酰基转移酶2(DiacylglycerolO-acyltransferase2,DGAT2)是植物中三羧酸甘油酯(TAG)合成途径的限速酶,其编码基因属于酰基转移酶基因超家族。本研究依托植物全基因组数据库Phytozome,通过BLAST搜索获得了蓖麻(RicinuscommunisL.)、拟南芥(ArabidopsisthalianaHeynh.)、毛果杨(Populustricho-carpaTorr.&A.Gray.)和木薯(ManihotesculentaCrantz.)4种双子叶植物酰基转移酶基因超家族所编码的73条多肽序列,并从中鉴定出5条DGAT2序列。理化性质和跨膜结构域分析表明,5条DGAT2序列均为疏水性跨膜蛋白,其中木薯DGAT2为一次跨膜蛋白且在叶绿体膜中大量分布,这与其他植物的DGAT2序列存在差异;木薯DGAT2蛋白在进化过程中发生了功能分化且可能与木薯的抗逆作用有关。

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