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枇杷幼果PLD和LOX对低温胁迫的响应

DOI: 10.11913/PSJ.2095-0837.2015.20203, PP. 203-209

Keywords: 枇杷,低温胁迫,磷脂酶D(PLD),脂氧合酶(LOX),膜结合Ca2+

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Abstract:

?以3年生枇杷品种‘早钟6号’(Eriobotryajaponica‘ZaozhongNo.6’)容器嫁接苗为试材,于0℃、-1℃、-3℃人工气候室内进行低温胁迫处理,探讨枇杷幼果细胞膜磷脂及相关酶对低温胁迫的响应机制。结果显示,在不同温度胁迫过程中,枇杷幼果磷脂酶D(PLD,EC3.1.4.4)和脂氧合酶(LOX,EC1.13.11.12)活性均呈上升趋势;质膜磷脂酰胆碱(PC)和磷脂酰肌醇(PI)含量因逐渐被降解而呈下降趋势,磷脂酸(PA)含量出现积累、增加,而膜结合Ca2+含量有不同程度的降低。随处理时间的延长和处理温度的降低,枇杷幼果细胞PLD和LOX活性增幅加大,从而加速了膜PC和PI的降解和PA的积累。低温胁迫过程中幼果细胞膜PC含量的降幅大于PI,膜结合Ca2+含量的变化与PLD和LOX活性变化呈负相关。低温胁迫下枇杷幼果细胞膜结合Ca2+含量的减少诱导了膜脂降解酶PLD和LOX活性的提高,并导致膜结构稳定性下降,加剧了低温胁迫对膜脂的降解和脂质过氧化伤害,其中尤以-3℃胁迫处理4~6h对幼果细胞质膜的伤害最严重。表明低温胁迫下Ca2+·CaM信使系统可能参与枇杷幼果细胞膜PLD和LOX活性的调控。

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