全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...

盐穗木钾转运蛋白基因HcKUP12的克隆及在盐胁迫下的表达分析

DOI: 10.11913/PSJ.2095-0837.2015.40499, PP. 499-506

Keywords: 盐穗木,钾转运蛋白基因HcKUP12,克隆,盐胁迫,表达分析

Full-Text   Cite this paper   Add to My Lib

Abstract:

?利用RACE技术从盐生植物盐穗木(Halostachyscaspica)中克隆获得了一个钾离子转运体基因,经BLAST同源比对发现该基因与拟南芥钾离子转运蛋白AtKUP12基因最为相似,命名为HcKUP12。HcKUP12基因cDNA全长为2953bp,含有2544bp的阅读框、262bp的5'-UTR和147bp的3'-UTR,编码847个氨基酸,分子质量为93.31kD,理论等电点为7.19。利用实时荧光定量RT-PCR分析了HcKUP12基因在600mmol/LNaCl处理下胁迫24h的表达模式,结果显示该基因在盐胁迫下表达量显著增加,至处理24h时达到最高(为对照的225倍),初步推测其可能是与盐穗木耐盐相关的一个候选基因。

References

[1]  杨荣超, 蔡玉静, 邓春婷, 欧阳波, 叶志彪. 番茄两个盐胁迫响应基因的cDNA克隆及其表达分析[J]. 植物科学学报, 2011, 29(2): 178-182.
[2]  刘洁, 胡蝶, 楚海家, 闫娟, 李建强. 花苜蓿抗旱耐盐EST-SSR标记筛选[J].植物科学学报, 2013, 31(5): 493-499.
[3]  Gierth M, Maser P. Potassium transporters in plants-involvement in K+ acquisition, redistribution and homeostasis[J]. FEBS Lett, 2007, 581(12): 2348-2356.
[4]  Hyun TK, Rim Y, Kim E, Kim JS. Genome-wide and molecular evolution analyses of the KT/HAK/KUP family in tomato (Solanum lycopersicum L.)[J]. Genes Genom, 2014, 3(36): 365-374.
[5]  Yang ZF, Gao QS, Sun CS, Li WJ, Gu SL, Xu CW. Molecular evolution and functional divergence of HAK potassium transporter gene family in rice (Oryza sativa L.)[J]. J Genet Genomics, 2009, 36(3): 161-172.
[6]  Gierth M, Maser P, Schroeder JI. The potassium transporter AtHAK5 functions in K+ deprivation-induced high-affinity K+ uptake and AKT1 K+ channel contribution to K+ uptake kinetics in Arabidopsis roots[J]. Plant Physiol, 2005, 137(3): 1105-1114.
[7]  马小娟, 戚金亮, 印莉萍, 黄勤妮. 植物钾离子转运相关蛋白及基因研究进展[J]. 首都师范大学学报:自然科学报, 2004, 25(2): 40-45.
[8]  Ashley MK, Grant M, Grabov A. Plant responses to potassium deficiencies: a role for potassium transport proteins[J]. J Exp Bot, 2006, 57(2): 425-436.
[9]  Mser P, Thomine S, Schroeder JI, Ward JM, Sze H, Talke IN, Hirschi K, Amtmann A, Maathuis FJ, Sanders D, Harper JF, Tchieu J, Gribskov M, Persans MW, Salt DE, Kim SA, Guerinot ML. Phylogenetic relationships within cation transporter families of Arabidopsis[J]. Plant Physiol, 2001, 126(4): 1646-1667.
[10]  Rubio F, Santa-María GE, Rodríguez-Navarro A. Cloning of Arabidopsis and barley cDNAs encoding HAK potassium transporters in root and shoot cells[J]. Physiol Plantarum, 2000, 1(109): 34-43.
[11]  宋志忠, 丛郁, 韩蕾, 王莉, 苏彦华. 葡萄基因组中KUP蛋白的生物信息学分析[J]. 基因组学与应用生物学, 2011, 6(30): 728-737.
[12]  鲁黎明. 主要农作物钾吸收转运基因及其进化关系分析[J]. 中国农业科学, 2009, 42(7): 2271-2279.
[13]  Osakabe Y, Arinaga N, Umezawa T, Katsura S, Nagamachi K, Tanaka H, Ohiraki H, Yamada K, Seo SU, Abo M, Yoshimura E, Yamaguchi-Shinozaki K, Shinozaki K. Osmotic stress responses and plant growth controlled by potassium transporters in Arabidopsis[J]. Plant Cell, 2013, 25(2): 609-624.
[14]  陶大勇. 盐穗木研究进展[J]. 安徽农业学报, 2007, 35(20): 6083-6084.
[15]  郗金标, 张福锁, 毛达如, 田长彦, 董振成, 王开芳. 新疆盐生植物群落物种多样性及其分布规律的初步研究[J]. 林业科学, 2006, 42(10): 6-12.
[16]  Liu L, Wang Y, Zeng YL, Haxim Y, Zhang FC. Identification and characterization of differentially expressed genes in the halophyte Halostachys caspica under salt stress[J]. Plant Cell Tiss Org, 2012, 110(1): 1-12.
[17]  鲁黎明, 杨铁钊. 烟草钾转运体基因NtHAK1的克隆及表达模式分析[J]. 核农学报, 2011, 25(3): 469-476.
[18]  Pyo YJ, Gierth M, Schroeder JI, Cho MH. High-affinity K+ transport in Arabidopsis: AtHAK5 and AKT1 are vital for seedling establishment and postgermination growth under low-potassium conditions[J]. Plant Physiol, 2010, 2(153): 863-875.
[19]  吴延寿, 陈春莲, 熊云华, 黄永萍, 周文红, 徐兰香, 尹建华. 植物体内Na/K转运体研究进展[J]. 江西农业学报, 2010, 22(6): 37-41.
[20]  Ahn SJ, Shin R, Schachtman DP. Expression of KT/KUP genes in Arabidopsis and the role of root hairs in K+ uptake[J]. Plant Physiol, 2004, 134(3): 1135-1145.
[21]  Gupta M, Qiu XH, Wang L, Xie WB, Zhang CJ, Xiong LZ, Lian XM, Zhang QF. KT/HAK/KUP potassium transporters gene family and their whole-life cycle expression profile in rice (Oryza sativa)[J]. Mol Genet Genomics, 2008, 5(280): 437-452
[22]  朱晓玲, 陈海峰, 王程, 郝青南, 陈李淼, 郭丹丹, 伍宝朵, 陈水莲, 沙爱华, 周蓉, 周新安. 大豆钾转运体基因GmKT12的克隆和信息学分析[J]. 作物学报, 2013, 39(9): 1701-1709.
[23]  Alakhverdiev SI, Sakamoto A, Nishiyama Y, lnaba M, Murata N. Ionic and osmotic effects of NaCl-induced inactivation of photosystems Ⅰ and Ⅱ in Synechococcus sp.[J]. Plant Physiol, 2000, 123(3): 1047-1056.
[24]  杨中敏, 王艳. 钾离子转运载体KUP/HAK/KT家族功能的研究进展[J]. 生物技术, 2014, 24(3): 100-103.
[25]  Nieves-Cordones M, Alemán F, Martínez V, Rubio F. The Arabidopsis thaliana HAK5 K+ transpor-ter is required for plant growth and K+ acquisition from low K solutions under saline conditions[J]. Mol Plant, 2010, 2(3): 326-333.

Full-Text

Contact Us

[email protected]

QQ:3279437679

WhatsApp +8615387084133