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大豆科学  2015 

锰胁迫对大豆氮代谢相关酶活性的影响

DOI: 10.11861/j.issn.1000-9841.2015.03.0432, PP. 432-435

Keywords: 锰胁迫,大豆,GS,GOGAT,GDH

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Abstract:

采用沙培的方法,以最适锰浓度为对照,设置重度锰缺乏(对照浓度的1/25,S1表示)、中度锰缺乏(对照浓度的1/5,S2表示)、中度锰过量(对照浓度的5倍,S3表示)、重度锰过量(对照浓度的25倍,S4表示)不同程度锰缺乏和锰过量处理,研究了锰胁迫对大豆氮代谢关键酶活性的影响。结果表明:不同程度锰缺乏胁迫抑制了叶片GS和GOGAT活性,而锰过量胁迫则起促进作用;锰缺乏胁迫对根GS无显著影响,锰过量胁迫则起抑制作用;不同程度锰胁迫对根GOGAT活性均起抑制作用,而对茎GS和GOGAT活性则无显著影响;重度锰胁迫(S1和S4)对叶片GDH起促进作用,轻度锰胁迫(S2和S3)起抑制作用;不同程度锰胁迫对茎和根GDH活性均起抑制作用。综合分析表明,大豆叶、茎、根GS、GOGAT、GDH活性对锰胁迫的响应不同,这可能与上述氮代谢相关酶在不同器官中的氮代谢功能、同工酶组成不同有关,并最终导致大豆氮代谢状况发生改变。

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